Caffeine and Ovariectomy Debilitate Bone Health in Rats on Calcium-deficient Diet.

IF 1.1 4区综合性期刊 Q3 MULTIDISCIPLINARY SCIENCES

Anais da Academia Brasileira de Ciencias Pub Date : 2024-08-05 eCollection Date: 2024-01-01 DOI:10.1590/0001-3765202420230095

Frederico S C Franco, Antônio José Natali, Neuza Maria B Costa

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Abstract

This study investigated the effects of ovariectomy and caffeine intake on bone health in rats on calcium-deficient diet. Forty adults female Wistar rats were divided into 4 groups in a 2x2 factorial design: Ovary (OVX/SHAM) and Caffeine (placebo/caffeine). The animals were housed in individual cages for 8 weeks, receiving 18-20g of AIN-93M diet per day, containing 50% of the daily recommended intake of calcium. The rats underwent ovariectomy (OVX) or laparotomy (SHAM) surgery. Caffeine groups received 6mg of caffeine/kg/day. After euthanasia, the tibia and femur were dissected to determine the calcium content and bone fracture strength, respectively. Blood sample was collected to determine serum Ostase. 24-hour urine was analyzed for excreted calcium and NTx. Reduced bone fracture strength and calcium content were observed in OVX and Caffeine groups. When observed separately, OVX group showed increased urinary NTx and lower bone weight, blood ostase, and urinary calcium. Caffeine groups showed elevated urinary calcium. There was a positive correlation between bone fracture strength and calcium content. NTx correlated negatively with bone calcium, fracture strength and thickness. In conclusion, both OVX and caffeine intake debilitate bone health in rats on calcium-deficient diet.

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咖啡因和卵巢切除术削弱了缺钙大鼠的骨骼健康

本研究调查了卵巢切除和咖啡因摄入对缺钙饮食大鼠骨骼健康的影响。40 只成年雌性 Wistar 大鼠被分成 4 组，采用 2x2 因式设计：卵巢组（OVX/SHAM）和咖啡因组（安慰剂/咖啡因）。这些动物被单独关在笼子里，为期 8 周，每天摄入 18-20 克 AIN-93M 食物，其中含钙量为每日推荐摄入量的 50%。大鼠接受卵巢切除术（OVX）或开腹手术（SHAM）。咖啡因组每天每公斤摄入 6 毫克咖啡因。安乐死后，解剖胫骨和股骨，分别测定钙含量和骨折强度。采集血样以测定血清奥斯特酶。分析24小时尿液中排出的钙和NTx。观察发现，OVX 组和咖啡因组的骨折强度和钙含量都有所降低。单独观察时，卵巢切除组显示尿中NTx增加，骨重、血液Ostase和尿钙降低。咖啡因组显示尿钙升高。骨折强度与钙含量呈正相关。NTx与骨钙、骨折强度和厚度呈负相关。总之，摄入卵巢切除术和咖啡因都会损害缺钙饮食大鼠的骨骼健康。

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来源期刊

Anais da Academia Brasileira de Ciencias 综合性期刊-综合性期刊

CiteScore

2.20

自引率

0.00%

发文量

347

审稿时长

1 months

期刊介绍： The Brazilian Academy of Sciences (BAS) publishes its journal, Annals of the Brazilian Academy of Sciences (AABC, in its Brazilianportuguese acronym ), every 3 months, being the oldest journal in Brazil with conkinuous distribukion, daking back to 1929. This scienkihic journal aims to publish the advances in scienkihic research from both Brazilian and foreigner scienkists, who work in the main research centers in the whole world, always looking for excellence. Essenkially a mulkidisciplinary journal, the AABC cover, with both reviews and original researches, the diverse areas represented in the Academy, such as Biology, Physics, Biomedical Sciences, Chemistry, Agrarian Sciences, Engineering, Mathemakics, Social, Health and Earth Sciences.

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