Exploring the Analgesic Initiation Mechanism of Tuina in the Dorsal Root Ganglion of Minor CCI Rats via the TRPV1/TRPA1-cGMP Pathway.

IF 2.5 3区 医学 Q2 CLINICAL NEUROLOGY
Pain Research & Management Pub Date : 2024-07-24 eCollection Date: 2024-01-01 DOI:10.1155/2024/2437396
Zhenjie Yang, Chula Sa, Tianyuan Yu, Jinping Chen, Runlong Zhang, Yingqi Zhang, Jiayue Liu, Hanyu Zhang, Jiawei Sun
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引用次数: 0

Abstract

Tuina is a treatment method in traditional Chinese medicine which has analgesic effects and effectively alleviates the symptoms of neuropathic pain (NP). Transient receptor potential vanilloid type 1 (TRPV1) and transient receptor potential ankyrin type 1 (TRPA1) play major roles in transmitting nociceptive sensory signals in the nociceptive primary sensory dorsal root ganglion (DRG) nerve. The nitric oxide (NO)/cyclic guanosine 3',5'-monophosphate(cGMP) pathway exerts both nociceptive and antinociceptive effects in various chronic pain models. TRPV1 and TRPA1 mediate the influx of calcium, which stimulates the generation of NO. Subsequently, NO activates the NO/cGMP/protein kinase G (PKG) signaling pathway, thereby improving hyperalgesia. In the present study, oa rat model of NP with minor chronic constriction injury (CCI) of the right sciatic nerve of NP was established. The results of behavioral testing showed that, after a one-time tuina intervention, the mechanical withdrawal threshold (MWT) and thermal withdrawal latency (TWL) were prolonged to varying degrees in the tuina group compared with the model group. Similarly, the expression of TRPV1, TRPA1, NO, soluble guanylate cyclase β (sGCβ), cGMP, and PKG1 was significantly decreased in the DRG of the tuina and tuina + TRPV1/TRPA1 antagonist group was significantly decreased. These findings suggest that the tuina intervention can effectively improve the symptoms of thermal and mechanical allodynia caused by peripheral nerve injuries. Tuina exerts immediate analgesic effects through the TRPV1/TRPA1-NO-cGMP-PKG signaling pathway.

探索推拿通过 TRPV1/TRPA1-cGMP 通路在 CCI 小鼠背根神经节中的镇痛启动机制
推拿是传统中医的一种治疗方法,具有镇痛作用,能有效缓解神经病理性疼痛(NP)的症状。瞬时受体电位类香草素 1 型(TRPV1)和瞬时受体电位淀粉样蛋白 1 型(TRPA1)在痛觉初级感觉背根神经节(DRG)神经的痛觉感觉信号传递中起主要作用。在各种慢性疼痛模型中,一氧化氮(NO)/环鸟苷-3',5'-单磷酸(cGMP)通路可产生痛觉和抗痛觉效应。TRPV1 和 TRPA1 介导钙的流入,从而刺激 NO 的生成。随后,NO 会激活 NO/cGMP/ 蛋白激酶 G(PKG)信号通路,从而改善痛觉减退。本研究建立了大鼠 NP 右坐骨神经轻微慢性收缩损伤(CCI)模型。行为测试结果表明,与模型组相比,经过一次性推拿干预后,推拿组大鼠的机械退缩阈值(MWT)和热退缩潜伏期(TWL)均有不同程度的延长。同样,推拿组和推拿+TRPV1/TRPA1拮抗剂组的DRG中TRPV1、TRPA1、NO、可溶性鸟苷酸环化酶β(sGCβ)、cGMP和PKG1的表达均显著降低。这些研究结果表明,推拿干预能有效改善周围神经损伤引起的热觉和机械异感症状。推拿通过TRPV1/TRPA1-NO-cGMP-PKG信号通路发挥即时镇痛作用。
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来源期刊
Pain Research & Management
Pain Research & Management CLINICAL NEUROLOGY-
CiteScore
5.30
自引率
0.00%
发文量
109
审稿时长
>12 weeks
期刊介绍: Pain Research and Management is a peer-reviewed, Open Access journal that publishes original research articles, review articles, and clinical studies in all areas of pain management. The most recent Impact Factor for Pain Research and Management is 1.685 according to the 2015 Journal Citation Reports released by Thomson Reuters in 2016.
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