Velocity of cerebrospinal fluid in the aqueduct measured by phase-contrast MRI in rat.

IF 2.7 4区医学 Q2 BIOPHYSICS

NMR in Biomedicine Pub Date : 2024-08-05 DOI:10.1002/nbm.5233

Guangliang Ding, Lian Li, Michael Chopp, Li Zhang, Qingjiang Li, Hao Luo, Min Wei, Jing Zhang, Edward Boyd, Zhenggang Zhang, Quan Jiang

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引用次数: 0

Abstract

Cerebrospinal fluid (CSF) circulation plays a key role in cerebral waste clearance via the glymphatic system. Although CSF flow velocity is an essential component of CSF dynamics, it has not been sufficiently characterized, and particularly, in studies of the glymphatic system in rat. To investigate the relationship between the flow velocity of CSF in the brain aqueduct and the glymphatic waste clearance rate, using phase-contrast MRI we performed the first measurements of CSF velocity in rats. Phase-contrast MRI was performed using a 7 T system to map mean velocity of CSF flow in the aqueduct in rat brain. The effects of age (3 months old versus 18 months old), gender, strain (Wistar, RNU, Dark Agouti), anesthetic agents (isoflurane versus dexmedetomidine), and neurodegenerative disorder (Alzheimer' disease in Fischer TgF344-AD rats, males and females) on CSF velocity were investigated in eight independent groups of rats (12 rats per group). Our results demonstrated that quantitative velocities of CSF flow in the aqueduct averaged 5.16 ± 0.86 mm/s in healthy young adult male Wistar rats. CSF flow velocity in the aqueduct was not altered by rat gender, strain, and the employed anesthetic agents in all rats, also age in the female rats. However, aged (18 months) Wistar male rats exhibited significantly reduced the CSF flow velocity in the aqueduct (4.31 ± 1.08 mm/s). In addition, Alzheimer's disease further reduced the CSF flow velocity in the aqueduct of male and female rats.

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通过相位对比核磁共振成像测量大鼠导水管中脑脊液的速度。

脑脊液（CSF）循环在通过甘液系统清除脑废物方面发挥着关键作用。虽然 CSF 流速是 CSF 动态变化的重要组成部分，但其特征还不够明显，尤其是在对大鼠甘回流系统的研究中。为了研究 CSF 在脑导水管中的流速与甘液废物清除率之间的关系，我们首次使用相位对比 MRI 对大鼠的 CSF 流速进行了测量。我们使用 7 T 系统进行了相位对比核磁共振成像，以绘制大鼠大脑导水管中 CSF 的平均流速图。我们在八组独立的大鼠（每组 12 只）中研究了年龄（3 个月大与 18 个月大）、性别、品系（Wistar、RNU、Dark Agouti）、麻醉剂（异氟烷与右美托咪定）和神经退行性疾病（Fischer TgF344-AD 大鼠中的阿尔茨海默病，雄性与雌性）对 CSF 流速的影响。我们的研究结果表明，健康的年轻成年雄性 Wistar 大鼠导水管中 CSF 的定量流速平均为 5.16 ± 0.86 mm/s。在所有大鼠中，导水管中的 CSF 流速不受大鼠性别、品系和所用麻醉剂的影响，雌性大鼠的年龄也是如此。然而，年龄较大（18 个月）的 Wistar 雄性大鼠导水管中的 CSF 流速明显降低（4.31 ± 1.08 mm/s）。此外，阿尔茨海默病进一步降低了雄性和雌性大鼠导水管中的 CSF 流速。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

NMR in Biomedicine 医学-光谱学

CiteScore

6.00

自引率

10.30%

发文量

209

审稿时长

3-8 weeks

期刊介绍： NMR in Biomedicine is a journal devoted to the publication of original full-length papers, rapid communications and review articles describing the development of magnetic resonance spectroscopy or imaging methods or their use to investigate physiological, biochemical, biophysical or medical problems. Topics for submitted papers should be in one of the following general categories: (a) development of methods and instrumentation for MR of biological systems; (b) studies of normal or diseased organs, tissues or cells; (c) diagnosis or treatment of disease. Reports may cover work on patients or healthy human subjects, in vivo animal experiments, studies of isolated organs or cultured cells, analysis of tissue extracts, NMR theory, experimental techniques, or instrumentation.