The G-protein alpha subunit AaGA1 positively regulates vegetative growth, appressorium-like formation, and pathogenicity in Alternaria alternata.

IF 3.2 3区 生物学 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY
Yuanping Nan, Miao Zhang, Yongcai Li, Yang Bi
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引用次数: 0

Abstract

Aims: The Gα subunit is a major component of heterotrimeric G proteins, which play a crucial role in the development and pathogenicity of several model fungi. However, its detailed function in the causal agent of pear black spot (Alternaria alternata) is unclear. Our aim was to understand the characteristics and functions of AaGA1 in A. alternata.

Methods and results: AaGA1 was cloned from A. alternata in this study, which encodes 353 amino acids and has a "G-alpha" domain. Mutant ΔAaGA1 resulted in reduced vegetative growth, conidiation, and spore germination. Especially, mutant ΔAaGA1 produced only fewer conidia on the V8A medium, and spore formation-related genes AbaA, BrlA, and WetA were significantly downregulated. More tolerance against cell wall-inhibiting agents was observed after the deletion of AaGA1. Moreover, AaGA1 deletion led to a significant reduction in melanin and toxin production. Interestingly, deletion of AaGA1 resulted in defective appressorium-like formations, complete loss of the ability to penetrate cellophane, and decreased infection on non-wound inoculated tobacco leaves. Cell wall-degrading enzyme-related genes PME, CL, Cut2, and LC were significantly downregulated in mutant ΔAaGA1 mutant, significantly reducing virulence on wound-inoculated pear fruits.

Conclusions: The G protein alpha subunit AaGA1 is indispensable for fungal development, appressorium-like formations, and pathogenicity in A. alternata.

G 蛋白α亚基 AaGA1 对交替孢霉的无性生长、附子体样形成和致病性具有正向调节作用。
目的:Gα 亚基是异源三聚体 G 蛋白的主要成分,在几种模式真菌的发育和致病性中起着至关重要的作用。然而,它在梨黑斑病(Alternaria alternata)病原菌中的具体功能尚不清楚。我们的目的是了解 AaGA1 在交替丝核菌中的特性和功能:本研究从交替孢霉中克隆了 AaGA1,它编码 353 个氨基酸,具有一个 "G-α "结构域。突变体ΔAaGA1导致无性生长、分生孢子和孢子萌发能力下降。特别是,突变体 ΔAaGA1 在 V8A 培养基上只产生较少的分生孢子,与孢子形成相关的基因 AbaA、BrlA 和 WetA 被显著下调。缺失 AaGA1 后,对细胞壁抑制剂的耐受性增强。此外,AaGA1 基因缺失导致黑色素和毒素的产生明显减少。有趣的是,缺失 AaGA1 会导致附着体形成缺陷,完全丧失穿透玻璃纸的能力,并减少对无伤口接种烟草叶片的感染。细胞壁降解酶相关基因 PME、CL、Cut2 和 LC 在突变体 ΔAaGA1 突变体中明显下调,显著降低了梨果伤口接种的毒力:结论:G 蛋白α亚基 AaGA1 对交替孢霉的真菌发育、附着体形成和致病性是不可或缺的。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
Journal of Applied Microbiology
Journal of Applied Microbiology 生物-生物工程与应用微生物
CiteScore
7.30
自引率
2.50%
发文量
427
审稿时长
2.7 months
期刊介绍: Journal of & Letters in Applied Microbiology are two of the flagship research journals of the Society for Applied Microbiology (SfAM). For more than 75 years they have been publishing top quality research and reviews in the broad field of applied microbiology. The journals are provided to all SfAM members as well as having a global online readership totalling more than 500,000 downloads per year in more than 200 countries. Submitting authors can expect fast decision and publication times, averaging 33 days to first decision and 34 days from acceptance to online publication. There are no page charges.
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