Induced Periodontitis in Rats With Three Ligature Types: An Exploratory Study

IF 1.7 Q3 DENTISTRY, ORAL SURGERY & MEDICINE

Clinical and Experimental Dental Research Pub Date : 2024-08-05 DOI:10.1002/cre2.946

Natalia Chatzaki, Andreas Stavropoulos, Balazs Denes, José Cancela, Stavros Kiliaridis, Catherine Giannopoulou

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Abstract

Background

The placement of ligatures in the cervical area of rat molars is considered as a predictable model to induce periodontitis.

Objectives

The present explorative study aimed to compare the efficacy of metal wires (MWs), without or with sandblasting, versus silk ligatures (SLs) in inducing periodontal bone loss in rats.

Materials and Methods

Twenty-four Wistar rats were randomly divided into three groups of eight rats that received three different types of ligatures (MW, sandblasted wire [SMW], and SL) around their first right mandibular molar, while the contralateral tooth was left without the ligature and served as a control. Bone loss was assessed by measuring the distance from the cementoenamel junction (CEJ) to the bone crest at the distal aspect of the first molar on central mesiodistal sections generated from micro-CT scans taken 24 and 35 days after ligature placement.

Results

In the SL group, only in two rats the ligatures were retained until the end of the 24-day period; in all other animals, the ligatures were lost at some time point. In the SMW, the ligatures were retained only for the 24-day period. In the MW group, no ligatures were lost. Irrespective of the group or experimental period, the difference in the crestal bone level between ligated and control teeth was in most cases z < 0.20 mm, that is, in 19 out of 25 pairs of teeth. In a few cases, the bone crest was more apically located at the control teeth compared to the ligated ones (four cases each, during both 24- and 35-day experimental periods).

Conclusions

Bone loss was minimal during the experimental period, with no significant differences between the test and control teeth, or among the three types of ligatures. MWs, not even roughened, do not seem to be a better alternative to SLs for inducing bone loss in the experimental periodontitis model in the rat. This assumption, however, has to be confirmed in a larger, well-powered study.

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用三种韧带类型诱发大鼠牙周炎：一项探索性研究

背景：在大鼠臼齿颈部放置结扎线被认为是诱导牙周炎的一种可预测模型：本探索性研究旨在比较不喷砂或喷砂金属丝（MW）与丝线结扎（SL）在诱导大鼠牙周骨质流失方面的功效：将 24 只 Wistar 大鼠随机分为三组，每组 8 只，分别在其第一颗右下颌臼齿周围接受三种不同类型的结扎（MW、喷砂丝 [SMW] 和 SL），而对侧牙齿则不接受结扎，作为对照。通过测量结扎后 24 天和 35 天显微 CT 扫描生成的第一磨牙远端中部牙周切面上牙本质牙釉质交界处（CEJ）到骨嵴的距离来评估骨质流失情况：结果：在SL组中，只有两只大鼠的结扎一直保留到24天结束；其他所有动物的结扎都在某个时间点丢失。在 SMW 组中，结扎只保留了 24 天。在 MW 组中，结扎没有丢失。无论在哪个组别或实验期间，被结扎牙齿与对照组牙齿之间的牙槽骨水平差异在大多数情况下都是 z 结论：在实验期间，骨质流失量很小，试验牙和对照牙之间以及三种结扎方式之间没有明显差异。在大鼠牙周炎实验模型中，在诱导骨质流失方面，MW（即使没有经过粗糙处理）似乎也不能更好地替代 SL。不过，这一假设还需要在更大规模、更有效的研究中得到证实。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Clinical and Experimental Dental Research DENTISTRY, ORAL SURGERY & MEDICINE-

CiteScore

3.30

自引率

5.60%

发文量

165

审稿时长

26 weeks

期刊介绍： Clinical and Experimental Dental Research aims to provide open access peer-reviewed publications of high scientific quality representing original clinical, diagnostic or experimental work within all disciplines and fields of oral medicine and dentistry. The scope of Clinical and Experimental Dental Research comprises original research material on the anatomy, physiology and pathology of oro-facial, oro-pharyngeal and maxillofacial tissues, and functions and dysfunctions within the stomatognathic system, and the epidemiology, aetiology, prevention, diagnosis, prognosis and therapy of diseases and conditions that have an effect on the homeostasis of the mouth, jaws, and closely associated structures, as well as the healing and regeneration and the clinical aspects of replacement of hard and soft tissues with biomaterials, and the rehabilitation of stomatognathic functions. Studies that bring new knowledge on how to advance health on the individual or public health levels, including interactions between oral and general health and ill-health are welcome.