Intracerebroventricular administration of the exercise hormone irisin or acute strenuous exercise alleviates epileptic seizure-induced neuroinflammation and improves memory dysfunction in rats.

IF 2.4 4区 医学 Q3 NEUROSCIENCES
Zarife Nigâr Ozdemir-Kumral, Tuğçe Akgün, Ceren Haşim, Ezgi Ulusoy, Mehmet Kaan Kalpakçıoğlu, Muhammet Ferzan Yüksel, Tunahan Okumuş, Zeynep Us, Dilek Akakın, Meral Yüksel, Zafer Gören, Berrak Ç Yeğen
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引用次数: 0

Abstract

Background: Status epilepticus is a common and potentially life-threatening neurological emergency with a high risk for cognitive and neurobiological impairment. Our aim was to evaluate the neuroprotective effects of centrally administered irisin and acute exhausting exercise against oxidative brain injury and memory dysfunction due to a pentylenetetrazole (PTZ)-induced single seizure. Male Sprague Dawley rats with intracerebroventricular (icv) cannulas were randomly divided into intraperitoneally (ip) saline-injected control and PTZ-injected (45 mg/kg) seizure groups. Both the control and PTZ groups were then treated with irisin (7.5 µg/kg, 2 µl, icv), saline (2 µl, icv) or were forced to an acute bout of strenuous exercise before the ip injection of saline (control) or PTZ. Seizures were evaluated using the Racine score. To evaluate memory performance, a passive avoidance test was performed before and after PTZ injection. Following euthanasia at the 24th hour of seizure induction, brain tissues were removed for histopathological examination and for evaluating oxidative damage, antioxidant capacity, and neurotransmitter levels.

Results: Glutamate/GABA imbalance observed in PTZ rats was corrected by irisin administration (p < 0.001/p < 0.01), while irisin prevented the generation of reactive oxygen species and lipid peroxidation (p < 0.05 - 0.001) and replenished the antioxidant catalase and glutathione levels (p < 0.01-0.01) in the cerebral tissue, and reduced the histologically evident neuronal injury due to a single seizure (p < 0.05 - 0.01). Irisin also delayed the onset of seizures (p < 0.05) and improved memory dysfunction (p < 0.05), but did not affect the severity of seizures. The acute exhaustive swimming exercise completed before PTZ-seizure depressed glutamate level (p < 0.001), maintained the oxidant/antioxidant balance, alleviated neuronal injury (p < 0.05 - 0.01) and upregulated cerebral BDNF expression (p < 0.05).

Conclusion: In conclusion, acute high-intensity exercise or exogenously administered irisin provides neuroprotection by maintaining the balance of excitatory/inhibitory neurotransmitters and oxidant/antioxidant systems.

脑室内注射运动荷尔蒙鸢尾素或急性剧烈运动可缓解癫痫发作诱发的神经炎症并改善大鼠的记忆功能障碍。
背景:癫痫状态是一种常见且可能危及生命的神经系统急症,极易造成认知和神经生物学损伤。我们的目的是评估中枢给药鸢尾素和急性疲劳运动对戊四唑(PTZ)诱导的单次癫痫发作引起的脑氧化损伤和记忆功能障碍的神经保护作用。雄性 Sprague Dawley 大鼠经脑室插管(icv)随机分为腹腔注射生理盐水对照组和注射 PTZ(45 毫克/千克)癫痫发作组。然后,对照组和 PTZ 组均接受鸢尾素(7.5 微克/千克,2 微升,icv)、生理盐水(2 微升,icv)治疗,或在 ip 注射生理盐水(对照组)或 PTZ 之前被迫进行急性剧烈运动。癫痫发作的评估采用拉辛评分法。为了评估记忆能力,在注射 PTZ 之前和之后进行了被动回避测试。在诱导癫痫发作的第24小时安乐死后,取出脑组织进行组织病理学检查,并评估氧化损伤、抗氧化能力和神经递质水平:结果:在 PTZ 大鼠体内观察到的谷氨酸/GABA 失衡通过服用鸢尾素得到了纠正(p 结论:鸢尾素对 PTZ 大鼠的神经递质水平具有显著的抑制作用:总之,急性高强度运动或外源性鸢尾素能维持兴奋/抑制神经递质和氧化/抗氧化系统的平衡,从而提供神经保护。
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来源期刊
BMC Neuroscience
BMC Neuroscience 医学-神经科学
CiteScore
3.90
自引率
0.00%
发文量
64
审稿时长
16 months
期刊介绍: BMC Neuroscience is an open access, peer-reviewed journal that considers articles on all aspects of neuroscience, welcoming studies that provide insight into the molecular, cellular, developmental, genetic and genomic, systems, network, cognitive and behavioral aspects of nervous system function in both health and disease. Both experimental and theoretical studies are within scope, as are studies that describe methodological approaches to monitoring or manipulating nervous system function.
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