Urinary anatalline and nicotelline cut-points to distinguish between exclusive and dual use of tobacco products.

IF 2 4区医学 Q3 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Biomarkers Pub Date : 2024-08-19 DOI:10.1080/1354750X.2024.2389047

Kathryn C Edwards, Gideon St Helen, Peyton Jacob, Jenny E Ozga, Cassandra A Stanton

{"title":"Urinary anatalline and nicotelline cut-points to distinguish between exclusive and dual use of tobacco products.","authors":"Kathryn C Edwards, Gideon St Helen, Peyton Jacob, Jenny E Ozga, Cassandra A Stanton","doi":"10.1080/1354750X.2024.2389047","DOIUrl":null,"url":null,"abstract":"Objective: This study measured anatalline and nicotelline, two minor tobacco alkaloids, to discriminate between exclusive smokeless tobacco (SLT) use, exclusive electronic nicotine delivery systems (ENDS) use, exclusive cigarette use, dual SLT and cigarette use, and dual ENDS and cigarette use.Methods: N = 664 urine samples from participants in the Population Assessment of Tobacco and Health Study were analyzed for anatalline and nicotelline. Geometric means and 95% confidence intervals were calculated for biomarker levels and their ratios. Non-parametric Receiver Operating Characteristic analyses were used to determine optimal cut-points of natural log-transformed biomarker ratios for distinguishing between tobacco use groups.Results: The anatalline/nicotelline ratio distinguished exclusive cigarette from exclusive SLT use (threshold = 18.1, sensitivity = 89.3%, specificity = 86.4%, AUC = 0.90), and exclusive SLT from exclusive ENDS use (threshold = 12.8, sensitivity = 96.4%, specificity = 76.3%, AUC = 0.90) very well, but had reduced sensitivity and specificity when distinguishing exclusive cigarette from exclusive ENDS or any dual use with cigarettes.Conclusions: This research fills a gap in understanding the public health consequences of SLT and ENDS use by providing objective measures that can signal use of these products alone or in combination with cigarettes.","PeriodicalId":8921,"journal":{"name":"Biomarkers","volume":null,"pages":null},"PeriodicalIF":2.0000,"publicationDate":"2024-08-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Biomarkers","FirstCategoryId":"3","ListUrlMain":"https://doi.org/10.1080/1354750X.2024.2389047","RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q3","JCRName":"BIOTECHNOLOGY & APPLIED MICROBIOLOGY","Score":null,"Total":0}

引用次数: 0

Abstract

Objective: This study measured anatalline and nicotelline, two minor tobacco alkaloids, to discriminate between exclusive smokeless tobacco (SLT) use, exclusive electronic nicotine delivery systems (ENDS) use, exclusive cigarette use, dual SLT and cigarette use, and dual ENDS and cigarette use.

Methods: N = 664 urine samples from participants in the Population Assessment of Tobacco and Health Study were analyzed for anatalline and nicotelline. Geometric means and 95% confidence intervals were calculated for biomarker levels and their ratios. Non-parametric Receiver Operating Characteristic analyses were used to determine optimal cut-points of natural log-transformed biomarker ratios for distinguishing between tobacco use groups.

Results: The anatalline/nicotelline ratio distinguished exclusive cigarette from exclusive SLT use (threshold = 18.1, sensitivity = 89.3%, specificity = 86.4%, AUC = 0.90), and exclusive SLT from exclusive ENDS use (threshold = 12.8, sensitivity = 96.4%, specificity = 76.3%, AUC = 0.90) very well, but had reduced sensitivity and specificity when distinguishing exclusive cigarette from exclusive ENDS or any dual use with cigarettes.

Conclusions: This research fills a gap in understanding the public health consequences of SLT and ENDS use by providing objective measures that can signal use of these products alone or in combination with cigarettes.

查看原文本刊更多论文

区分烟草制品专用和双重用途的尿液锐毒碱和烟碱切点。

研究目的本研究测定了两种次要烟草生物碱--锐他林和烟碱，以区分无烟烟草（SLT）的唯一使用、电子尼古丁释放系统（ENDS）的唯一使用、卷烟的唯一使用、SLT和卷烟的双重使用以及ENDS和卷烟的双重使用：对烟草与健康人群评估研究参与者的 N = 664 份尿样进行了锐毒碱和烟碱分析。计算了生物标志物水平及其比率的几何平均数和 95% 置信区间。使用非参数接收者操作特征分析确定自然对数转换生物标志物比率的最佳切点，以区分烟草使用群体：结果：锐钛碱/烟碱比值能很好地区分香烟和SLT的独占使用（阈值=18.1，灵敏度=89.3%，特异性=86.4%，AUC=0.90），以及SLT和ENDS的独占使用（阈值=12.8，灵敏度=96.4%，特异性=76.3%，AUC=0.90），但在区分香烟和ENDS的独占使用或任何与香烟的双重使用时，灵敏度和特异性都有所降低：这项研究填补了了解使用 SLT 和 ENDS 的公共卫生后果方面的空白，提供了客观的测量方法，可以显示这些产品单独使用或与香烟混合使用的情况。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文求助全文

来源期刊

Biomarkers 医学-毒理学

CiteScore

5.00

自引率

3.80%

发文量

140

审稿时长

3 months

期刊介绍： The journal Biomarkers brings together all aspects of the rapidly growing field of biomarker research, encompassing their various uses and applications in one essential source. Biomarkers provides a vital forum for the exchange of ideas and concepts in all areas of biomarker research. High quality papers in four main areas are accepted and manuscripts describing novel biomarkers and their subsequent validation are especially encouraged: • Biomarkers of disease • Biomarkers of exposure • Biomarkers of response • Biomarkers of susceptibility Manuscripts can describe biomarkers measured in humans or other animals in vivo or in vitro. Biomarkers will consider publishing negative data from studies of biomarkers of susceptibility in human populations.