The effect of light availability and light wavelength on growth, 2-MIB biosynthesis, and 2-MIB-related gene expression in Pseudanabaena foetida var. intermedia

IF 4.6 Q2 MATERIALS SCIENCE, BIOMATERIALS
Kaushalya Dayarathne, Toshiki Ishikawa, Aikeranmu Kadeer, Masatoshi Yamaguchi, Maki Kawai-Yamada
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引用次数: 0

Abstract

2-methylisoborneol (2-MIB) is an odiferous metabolite mainly produced by cyanobacteria, contributing to taste and odor problems in drinking water. The mechanisms involved in 2-MIB biosynthesis in cyanobacteria are not yet completely understood. This study investigated the effect of light availability and wavelength on growth, 2-MIB synthesis, and related gene expression in Pseudanabaena foetida var. intermedia. A significantly lower 2-MIB production was observed in P. foetida var. intermedia during the dark period of a 12-h photoperiod. Exposure to green light resulted in a significant decrease in 2-MIB production compared to white light and red light. The relative expression levels of 2-MIB-related genes in P. foetida var. intermedia were significantly lower during the dark period of a 12-h photoperiod and when cultured under green light. The expression of 2-MIB-related genes in cyanobacteria appears to be light-dependent. This study suggests that the demand for photopigment synthesis under unfavorable light conditions affects the 2-MIB synthesis in cyanobacteria.

Abstract Image

Abstract Image

光照条件和光波长对福氏拟尾柱虫变种生长、2-MIB 生物合成和 2-MIB 相关基因表达的影响
2- 甲基异龙脑(2-MIB)是一种有异味的代谢物,主要由蓝藻产生,造成饮用水的味道和气味问题。蓝藻中 2-MIB 的生物合成机制尚不完全清楚。本研究调查了光照和波长对 Pseudanabaena foetida var.在 12 小时光周期的黑暗期,P. foetida var. intermedia 的 2-MIB 产量明显较低。与白光和红光相比,绿光导致 2-MIB 生成量明显减少。在 12 小时光周期的黑暗期和绿光下培养时,P. foetida var. intermedia 中 2-MIB 相关基因的相对表达水平明显较低。蓝藻中 2-MIB 相关基因的表达似乎与光照有关。这项研究表明,在不利的光照条件下,对光色素合成的需求会影响蓝藻中 2-MIB 的合成。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
ACS Applied Bio Materials
ACS Applied Bio Materials Chemistry-Chemistry (all)
CiteScore
9.40
自引率
2.10%
发文量
464
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