Metagenomic Insights Reveal Unrecognized Diversity of Entotheonella in Japanese Theonella Sponges

IF 2.6 3区 生物学 Q3 BIOTECHNOLOGY & APPLIED MICROBIOLOGY
Sota Yamabe, Kazutoshi Yoshitake, Akihiro Ninomiya, Jörn Piel, Haruko Takeyama, Shigeki Matsunaga, Kentaro Takada
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Abstract

Numerous biologically active natural products have been discovered from marine sponges, particularly from Theonella swinhoei, which is known to be a prolific source of natural products such as polyketides and peptides. Recent studies have revealed that many of these natural products are biosynthesized by Candidatus Entotheonella phylotypes, which are uncultivated symbionts within T. swinhoei. Consequently, Entotheonella is considered an untapped biochemical resource. In this study, we conducted metagenomic analyses to assess the diversity of Entotheonella in two T. swinhoei Y and two T. swinhoei W (Y and W referring to the yellow and white interior of the sponge, respectively), after separating filamentous bacteria using density gradient centrifugation. We obtained five Entotheonella metagenome-assembled genomes (MAGs) from filamentous bacteria-enriched fractions. Notably, one of these MAGs is significantly different from previously reported Entotheonella variants. Additionally, we identified closely related Entotheonella members present across different chemotypes of T. swinhoei. Thus, our metagenomic insights reveal that the diversity of Entotheonella within Theonella sponges is greater than previously recognized.

Abstract Image

Abstract Image

元基因组研究揭示了日本海螺海绵中尚未认识到的 Entotheonella 多样性。
人们从海洋海绵中发现了许多具有生物活性的天然产品,尤其是从众所周知的天然产品(如多酮类和肽类)来源丰富的黑头褐藻(Theonella swinhoei)中。最近的研究发现,这些天然产物中有许多是由黑头栉水母菌属(Candidatus Entotheonella phylotypes)生物合成的,黑头栉水母菌属是黑头栉水母内未经培养的共生菌。因此,Entotheonella 被认为是一种尚未开发的生化资源。在本研究中,我们利用密度梯度离心分离丝状菌后,进行了元基因组分析,以评估两块T. swinhoei Y和两块T. swinhoei W(Y和W分别指海绵的黄色和白色内部)中的 Entotheonella 的多样性。我们从丝状菌富集的馏分中获得了五个神仙掌元基因组(MAGs)。值得注意的是,其中一个 MAG 与之前报道的 Entotheonella 变异体有显著不同。此外,我们还发现了在 T. swinhoei 的不同化学型中存在的密切相关的 Entotheonella 成员。因此,我们的元基因组研究揭示了海绵栉水母内 Entotheonella 的多样性比以前认识到的要大。
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来源期刊
Marine Biotechnology
Marine Biotechnology 工程技术-海洋与淡水生物学
CiteScore
4.80
自引率
3.30%
发文量
95
审稿时长
2 months
期刊介绍: Marine Biotechnology welcomes high-quality research papers presenting novel data on the biotechnology of aquatic organisms. The journal publishes high quality papers in the areas of molecular biology, genomics, proteomics, cell biology, and biochemistry, and particularly encourages submissions of papers related to genome biology such as linkage mapping, large-scale gene discoveries, QTL analysis, physical mapping, and comparative and functional genome analysis. Papers on technological development and marine natural products should demonstrate innovation and novel applications.
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