Analysis of histomorphology and SERNINA5 gene expression in different regions of epididymis of cattleyak

IF 2.9 4区生物学 Q3 CELL BIOLOGY

Journal of Molecular Histology Pub Date : 2024-08-06 DOI:10.1007/s10735-024-10234-0

Haiyan Li, Cheng Pan, Yifei Wang, Jingjing Li, Zhenzhen Zhang, Khuram Shahzad, Shehr Bano Mustafa, Ye Wang, Wangsheng Zhao

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引用次数: 0

Abstract

The molecular mechanism of sterility in cattleyak is still unresolved. The related factors of infertility in cattleyak were studied by tissue section, SERPINA5 gene cloning and bioinformatics technology. Tissue sections of the epididymis showed poorly structured and disorganized epithelial cells in the corpus of the epididymis compared to the caput of the epididymis, while in the cauda part of the epididymis, the extra basal smooth muscle was thinner, the surface of the epithelial lumen was discontinuous and the epithelium was markedly degenerated. The results of gene cloning showed that the coding sequence (CDS) region of the SERPINA5 gene in cattleyak was 1215 bp in length, encoding a total of 404 amino acids, of which the isoleucine content was the highest, accounting for a total of 49 amino acids (12.1%). The results of real-time fluorescence quantitative PCR (qPCR) showed that the expression of the SERPINA5 gene in the epididymis caput in cattleyak was significantly higher than that in the corpus and cauda (P < 0.05), but there were no significant differences between the corpus and cauda. In the current study, histological and bioinformatics analysis, physicochemical properties, and the expression analysis of the SERPINA5 gene in different regions of the epididymis in cattleyak were carried out to explore the biological complications of cattleyak infertility.

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分析卡特兰牦牛附睾不同区域的组织形态和 SERNINA5 基因表达。

卡特兰牦牛不育症的分子机制仍未得到解决。通过组织切片、SERPINA5基因克隆和生物信息学技术研究了卡特兰牦牛不育的相关因素。附睾组织切片显示，与附睾头相比，附睾体的上皮细胞结构较差且杂乱无章，而在附睾尾部，基底外平滑肌较薄，上皮腔表面不连续，上皮明显退化。基因克隆结果表明，卡特兰牦牛 SERPINA5 基因的编码序列（CDS）区长度为 1215 bp，共编码 404 个氨基酸，其中异亮氨酸含量最高，共占 49 个氨基酸（12.1%）。实时荧光定量 PCR（qPCR）结果显示，卡特兰牦牛附睾睾丸中 SERPINA5 基因的表达量明显高于冠状沟和尾状沟（P＜0.05）。

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来源期刊

Journal of Molecular Histology 生物-细胞生物学

CiteScore

5.90

自引率

0.00%

发文量

审稿时长

1 months

期刊介绍： The Journal of Molecular Histology publishes results of original research on the localization and expression of molecules in animal cells, tissues and organs. Coverage includes studies describing novel cellular or ultrastructural distributions of molecules which provide insight into biochemical or physiological function, development, histologic structure and disease processes. Major research themes of particular interest include: - Cell-Cell and Cell-Matrix Interactions; - Connective Tissues; - Development and Disease; - Neuroscience. Please note that the Journal of Molecular Histology does not consider manuscripts dealing with the application of immunological or other probes on non-standard laboratory animal models unless the results are clearly of significant and general biological importance. The Journal of Molecular Histology publishes full-length original research papers, review articles, short communications and letters to the editors. All manuscripts are typically reviewed by two independent referees. The Journal of Molecular Histology is a continuation of The Histochemical Journal.