CBX4/miR-190 regulatory loop inhibits lung cancer metastasis.

IF 2.3 3区医学 Q3 ONCOLOGY

Thoracic Cancer Pub Date : 2024-09-01 Epub Date: 2024-08-04 DOI:10.1111/1759-7714.15415

Jian Wang, Xiang Zhu, Yue Yu, Jie Ge, Wei Chen, Wengui Xu, Wen Zhou

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引用次数: 0

Abstract

Background: Lung cancer is one of the major threats to human life worldwide. MiR-190 has been found to perform essential roles in multiple cancer progression; however, there have been no studies focused on its function and underlying regulatory mechanism in lung cancer.

Method: The miR-190 expression was detected by real-time quantitative polymerase chain reaction (RT-qPCR). The cell functional experiments, including cell counting kit-8 (CCK-8), colony formation and transwell assay were conducted in vitro, as well as animal experiments performed in vivo. The regulation and potential binding sites of CBX4 on miR-190 were predicted by TCGA data set and JASPAR website and verified by ChIP assay and dual-luciferase reporter assay. The prospects binding site of miR-190-3p on CBX4 3'UTR region was predicted by StarBase and verified by dual-luciferase reporter assay.

Results: MiR-190 was decreased in lung cancer cells. The overexpression of miR-190 had no effects on cell proliferation, but significantly inhibited cancer metastasis both in vitro and in vivo. Moreover, miR-190 expression could be transcriptionally inhibited by CBX4, and CBX4 was the direct target of miR-190-3p.

Conclusion: MiR-190 served as a cancer metastasis inhibitor in lung cancer and formed a regulatory loop with CBX4. These findings provided emerging insights into therapeutic targets and strategies for metastatic lung cancer.

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CBX4/miR-190调节环抑制肺癌转移

背景：肺癌是全球威胁人类生命的主要疾病之一。研究发现，miR-190 在多种癌症进展过程中发挥着重要作用，但目前还没有关于其在肺癌中的功能和潜在调控机制的研究：方法：采用实时定量聚合酶链反应（RT-qPCR）检测 miR-190 的表达。方法：采用实时定量聚合酶链式反应（RT-qPCR）检测 miR-190 的表达，并在体外进行细胞功能实验，包括细胞计数试剂盒-8（CCK-8）、集落形成和透孔实验，以及在体内进行动物实验。通过 TCGA 数据集和 JASPAR 网站预测了 CBX4 对 miR-190 的调控和潜在结合位点，并通过 ChIP 检测和双荧光素酶报告实验进行了验证。通过StarBase预测了miR-190-3p在CBX4 3'UTR区域的前景结合位点，并通过双荧光素酶报告实验进行了验证：结果：MiR-190在肺癌细胞中的表达量减少。结果：肺癌细胞中的 miR-190 表达量减少，过表达 miR-190 对细胞增殖无影响，但能显著抑制体外和体内的癌细胞转移。此外，miR-190的表达可被CBX4转录抑制，而CBX4是miR-190-3p的直接靶标：结论：miR-190 是肺癌的癌症转移抑制因子，并与 CBX4 形成了一个调控环。这些发现为转移性肺癌的治疗靶点和策略提供了新的见解。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Thoracic Cancer ONCOLOGY-RESPIRATORY SYSTEM

CiteScore

5.20

自引率

3.40%

发文量

439

审稿时长

2 months

期刊介绍： Thoracic Cancer aims to facilitate international collaboration and exchange of comprehensive and cutting-edge information on basic, translational, and applied clinical research in lung cancer, esophageal cancer, mediastinal cancer, breast cancer and other thoracic malignancies. Prevention, treatment and research relevant to Asia-Pacific is a focus area, but submissions from all regions are welcomed. The editors encourage contributions relevant to prevention, general thoracic surgery, medical oncology, radiology, radiation medicine, pathology, basic cancer research, as well as epidemiological and translational studies in thoracic cancer. Thoracic Cancer is the official publication of the Chinese Society of Lung Cancer, International Chinese Society of Thoracic Surgery and is endorsed by the Korean Association for the Study of Lung Cancer and the Hong Kong Cancer Therapy Society. The Journal publishes a range of article types including: Editorials, Invited Reviews, Mini Reviews, Original Articles, Clinical Guidelines, Technological Notes, Imaging in thoracic cancer, Meeting Reports, Case Reports, Letters to the Editor, Commentaries, and Brief Reports.