Protein Network Alterations in G-CSF Treated Severe Congenital Neutropenia Patients and Beneficial Effects of Oral Health Intervention.

IF 2.1 4区生物学 Q3 BIOCHEMICAL RESEARCH METHODS

PROTEOMICS – Clinical Applications Pub Date : 2024-11-01 Epub Date: 2024-08-03 DOI:10.1002/prca.202400064

Kai Bao, Angelika Silbereisen, Jonas Grossmann, Paolo Nanni, Peter Gehrig, Gülnur Emingil, Merve Erguz, Deniz Yilmaz Karapinar, Burç Pekpinarli, Georgios N Belibasakis, Georgios Tsilingaridis, Egija Zaura, Nagihan Bostanci

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引用次数: 0

Abstract

Purpose: Severe congenital neutropenia (SCN) is a raredisorder characterized by diminished neutrophil levels. Despite granulocytecolony-stimulating factor (G-CSF) treatment, SCN patients remain still prone tosevere infections, including periodontal disease-a significant oral healthrisk. This study investigates the host proteome and metaproteome in saliva andgingival crevicular fluid (GCF) of G-CSF-treated patients.

Experimental design: We used label-free quantitative proteomics on saliva and GCF samples from SCN patients before (n = 10, mean age: 10.7 ± 6.6 years) and after a 6-month oral hygiene intervention (n = 9,mean age: 11.6 ± 5.27 years), and from 12 healthy controls.

Results: We quantified 894 proteins in saliva (648 human,246 bacterial) and 756 proteins in GCF (493 human, 263 bacterial). Predominant bacterial genera included Streptococcus, Veillonella, Selenomonas, Corynebacterium, Porphyromonas, and Prevotella. SCN patients showed reduced antimicrobial peptides (AMPs) and elevated complement proteins compared tohealthy controls. Oral hygiene intervention improved oral epithelial conditionsand reduced both AMPs and complement proteins.

Conclusions and clinical relevance: SCN patients have aunique proteomic profile with reduced AMPs and increased complement proteins, contributing to infection susceptibility. Oral hygiene intervention not onlyimproved oral health in SCN patients but also offers potential overall therapeuticbenefits.

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经 G-CSF 治疗的重度先天性中性粒细胞减少症患者的蛋白质网络变化及口腔健康干预的有益影响

目的：严重先天性中性粒细胞减少症（SCN）是一种以中性粒细胞水平降低为特征的罕见疾病。尽管接受了粒细胞集落刺激因子（G-CSF）治疗，重症先天性中性粒细胞减少症患者仍然容易发生严重感染，包括牙周病--这是一种严重的口腔健康风险。本研究调查了接受过 G-CSF 治疗的患者唾液和牙龈缝隙液（GCF）中的宿主蛋白质组和元蛋白质组：实验设计：我们采用无标记定量蛋白质组学方法对 SCN 患者（10 人，平均年龄为 10.7 ± 6.6 岁）在接受 6 个月口腔卫生干预（9 人，平均年龄为 11.6 ± 5.27 岁）之前和之后的唾液和 GCF 样本以及 12 名健康对照者的唾液和 GCF 样本进行了研究：我们对唾液中的 894 种蛋白质（648 种人类蛋白质，246 种细菌蛋白质）和 GCF 中的 756 种蛋白质（493 种人类蛋白质，263 种细菌蛋白质）进行了量化。主要细菌属包括链球菌、Veillonella、Selenomonas、Corynebacterium、Porphyromonas 和 Prevotella。与健康对照组相比，SCN 患者的抗菌肽 (AMP) 减少，补体蛋白升高。口腔卫生干预改善了口腔上皮状况，降低了抗菌肽和补体蛋白：SCN 患者具有独特的蛋白质组学特征，AMPs 减少，补体蛋白增加，导致感染易感性。口腔卫生干预不仅能改善 SCN 患者的口腔健康，还能带来潜在的整体治疗效果。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

PROTEOMICS – Clinical Applications 医学-生化研究方法

CiteScore

5.20

自引率

5.00%

发文量

审稿时长

1 months

期刊介绍： PROTEOMICS - Clinical Applications has developed into a key source of information in the field of applying proteomics to the study of human disease and translation to the clinic. With 12 issues per year, the journal will publish papers in all relevant areas including: -basic proteomic research designed to further understand the molecular mechanisms underlying dysfunction in human disease -the results of proteomic studies dedicated to the discovery and validation of diagnostic and prognostic disease biomarkers -the use of proteomics for the discovery of novel drug targets -the application of proteomics in the drug development pipeline -the use of proteomics as a component of clinical trials.