The effect of Andaliman (Zanthoxylum acanthopodium DC.) fruit extracted with ethanol on TNF-α and TRPA-1 levels in type II diabetes-induced mice.

IF 1.5 Q3 AGRICULTURE, DAIRY & ANIMAL SCIENCE
Journal of Advanced Veterinary and Animal Research Pub Date : 2024-06-06 eCollection Date: 2024-06-01 DOI:10.5455/javar.2024.k774
Boyke Marthin Simbolon, O K Yulizal, Albert Manggading Hutapea, Erwin Handoko
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Abstract

Objective: The present study investigated the effects of Andaliman fruit extract on tumor necrosis factor-alpha (TNF-α) and transient receptor potential ankyrin-1 (TRPA-1) levels in type 2 diabetes mellitus (T2DM) mouse models induced with streptozocin (STZ) and a high-fat diet (HFD).

Materials and methods: In this research, mice were allocated into six distinct groups: normal, negative control (HFD and STZ), positive control (metformin, HFD, and STZ), and three treatment groups (HFD, STZ, and Andaliman extract at varying dosages of 100, 300, and 500 mg/kg, respectively). Body weight and blood glucose levels (BGLs) were recorded at weeks 1 (baseline), 8, 12, and 16. The levels of TNF-α and TRPA-1 were measured during the 16th week.

Results: Phytochemical screening of the Andaliman extract revealed the presence of flavonoids, alkaloids, tannins, saponins, and glycosides. The one-way ANOVA revealed significantly elevated BGL at week 16 in the negative control group in comparison to the other groups (p < 0.05). The Kruskal-Wallis test followed by Bonferroni-corrected pairwise comparisons showed that the negative control had significantly higher TNF-α levels than the Andaliman-groups (z = 22.11, p < 0.01). TRPA-1 was significantly higher in the negative control group compared to the treatment groups (p < 0.05). Furthermore, Spearman's rho analysis revealed a statistically significant positive association between BGL and both TNF-α and TRPA-1, as well as between TNF-α and TRPA.

Conclusion: Andaliman extract potentially serves as a therapy for diabetic neuropathy in T2DM by lowering BGL and inhibiting the expression of TNF-α and TRPA-1.

乙醇提取的安达利曼(Zanthoxylum acanthopodium DC.)果实对 II 型糖尿病诱导小鼠 TNF-α 和 TRPA-1 水平的影响
研究目的本研究探讨了安达利曼果提取物对链佐辛(STZ)和高脂饮食(HFD)诱导的2型糖尿病(T2DM)小鼠模型中肿瘤坏死因子-α(TNF-α)和瞬时受体电位ankyrin-1(TRPA-1)水平的影响:本研究将小鼠分为六个不同的组别:正常组、阴性对照组(HFD 和 STZ)、阳性对照组(二甲双胍、HFD 和 STZ)以及三个治疗组(HFD、STZ 和安达利曼提取物,剂量分别为 100、300 和 500 mg/kg)。分别在第1周(基线)、第8周、第12周和第16周记录体重和血糖水平(BGLs)。第 16 周测量 TNF-α 和 TRPA-1 的水平:结果:安达利曼提取物的植物化学筛选显示,其中含有黄酮类、生物碱、鞣质、皂苷和苷类。单因素方差分析显示,与其他组相比,阴性对照组在第 16 周的血糖胆固醇水平明显升高(p < 0.05)。Kruskal-Wallis 检验和 Bonferroni 校正配对比较显示,阴性对照组的 TNF-α 水平明显高于安达利曼组(z = 22.11,P 0.01)。与治疗组相比,阴性对照组的 TRPA-1 水平明显更高(p < 0.05)。此外,Spearman's rho 分析显示,BGL 与 TNF-α 和 TRPA-1 之间以及 TNF-α 与 TRPA 之间均存在统计学意义上的显著正相关:结论:安达利曼提取物可通过降低血糖胆固醇水平、抑制 TNF-α 和 TRPA-1 的表达来治疗 T2DM 中的糖尿病神经病变。
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来源期刊
CiteScore
2.70
自引率
7.10%
发文量
41
审稿时长
6 weeks
期刊介绍: Journal of Advanced Veterinary and Animal Research (JAVAR) - is an open access, international, peer-reviewed, quarterly, highly-indexed scientific journal publishing original research findings and reviews on all aspects of veterinary and animal sciences. Basic and applied researches on- - Anatomy & histology - Animal health economics - Animal nutrition - Animal reproduction - Animal science - Antimicrobial resistance (AMR) - Biochemistry - Biotechnology - Dairy science - Epidemiology - Food hygiene and technology - Genetics and breeding - Immunology - Microbiology - Parasitology - Pathology - Pharmacology & toxicology - Physiology - Poultry science - Preventive veterinary medicine - Public health - Surgery & obstetrics - Veterinary extension studies - Wildlife & aquatic medicine - Zoo animal medicine.
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