Molecular detection and genotyping of bovine viral diarrhea virus in Selangor, Malaysia.

IF 16.4 1区化学 Q1 CHEMISTRY, MULTIDISCIPLINARY

Accounts of Chemical Research Pub Date : 2024-06-16 eCollection Date: 2024-06-01 DOI:10.5455/javar.2024.k797

Nurulhidayah Khalid, Siti Suri Arshad, Nurhusien Yimer Degu, Siti Zubaidah Ramanoon, Mohammed Babatunde Sadiq

{"title":"Molecular detection and genotyping of bovine viral diarrhea virus in Selangor, Malaysia.","authors":"Nurulhidayah Khalid, Siti Suri Arshad, Nurhusien Yimer Degu, Siti Zubaidah Ramanoon, Mohammed Babatunde Sadiq","doi":"10.5455/javar.2024.k797","DOIUrl":null,"url":null,"abstract":"Objective: Bovine viral diarrhea (BVD) disease is a viral infection in cows caused by a single-stranded plus-sense RNA virus of the Pestivirus genus under the Flaviviridae family. The clinical manifestation of BVD mainly includes diarrhea and immunosuppression, thereby exacerbating various respiratory diseases. This study was conducted to detect and molecularly characterize the bovine viral diarrhea disease virus (BVDV) in cattle on selected farms in Selangor, Malaysia.Materials and methods: A reverse transcription polymerase chain reaction (RT-PCR) was performed for antigen detection in 253 plasma samples collected from cows using a cross-sectional study design. We selected the 5 untranslated regions (5'-UTR) region and the E2 region to compare the genetic differences between the isolates.Results: One sample was found to be positive (1/253) following RT-PCR targeting the conserved 5'-UTR region of BVDV. Thus, BVDV antigen prevalence was 0.40% (95% confidence interval: 0.0%-2.2%). By targeting the hypervariable E2 region of the isolated virus, UPM/MAL/BVDV/D17, the virus was classified under the subgenotype BVDV-1a.Conclusion: BVDV is present and circulating on selected cattle farms in Selangor, Malaysia. Given the presence of BVDV in several subgenotypes, the screening of all incoming cattle at Malaysia's border is pertinent to prevent the entry of other BVDV subgenotypes into the country.","PeriodicalId":1,"journal":{"name":"Accounts of Chemical Research","volume":null,"pages":null},"PeriodicalIF":16.4000,"publicationDate":"2024-06-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11296188/pdf/","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Accounts of Chemical Research","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.5455/javar.2024.k797","RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"2024/6/1 0:00:00","PubModel":"eCollection","JCR":"Q1","JCRName":"CHEMISTRY, MULTIDISCIPLINARY","Score":null,"Total":0}

引用次数: 0

Abstract

Objective: Bovine viral diarrhea (BVD) disease is a viral infection in cows caused by a single-stranded plus-sense RNA virus of the Pestivirus genus under the Flaviviridae family. The clinical manifestation of BVD mainly includes diarrhea and immunosuppression, thereby exacerbating various respiratory diseases. This study was conducted to detect and molecularly characterize the bovine viral diarrhea disease virus (BVDV) in cattle on selected farms in Selangor, Malaysia.

Materials and methods: A reverse transcription polymerase chain reaction (RT-PCR) was performed for antigen detection in 253 plasma samples collected from cows using a cross-sectional study design. We selected the 5 untranslated regions (5'-UTR) region and the E2 region to compare the genetic differences between the isolates.

Results: One sample was found to be positive (1/253) following RT-PCR targeting the conserved 5'-UTR region of BVDV. Thus, BVDV antigen prevalence was 0.40% (95% confidence interval: 0.0%-2.2%). By targeting the hypervariable E2 region of the isolated virus, UPM/MAL/BVDV/D17, the virus was classified under the subgenotype BVDV-1a.

Conclusion: BVDV is present and circulating on selected cattle farms in Selangor, Malaysia. Given the presence of BVDV in several subgenotypes, the screening of all incoming cattle at Malaysia's border is pertinent to prevent the entry of other BVDV subgenotypes into the country.

查看原文本刊更多论文

马来西亚雪兰莪州牛病毒性腹泻病毒的分子检测和基因分型。

目的：牛病毒性腹泻病（BVD）是由一种单链加有义 RNA 病毒引起的奶牛病毒性传染病。BVD 的临床表现主要包括腹泻和免疫抑制，从而加重各种呼吸道疾病。本研究旨在检测马来西亚雪兰莪州部分农场牛群中的牛病毒性腹泻病病毒（BVDV），并对其进行分子鉴定：采用横断面研究设计，对从奶牛身上采集的 253 份血浆样本进行反转录聚合酶链反应（RT-PCR）以检测抗原。我们选择了 5 个非翻译区（5'-UTR）区域和 E2 区域，以比较不同分离株之间的基因差异：结果：在针对 BVDV 5'-UTR 保守区的 RT-PCR 检测中，发现一个样本呈阳性（1/253）。因此，BVDV 抗原流行率为 0.40%（95% 置信区间：0.0%-2.2%）。针对分离出的病毒 UPM/MAL/BVDV/D17 的超变异 E2 区，该病毒被归类为 BVDV-1a 亚基因型：结论：BVDV 在马来西亚雪兰莪州的部分养牛场存在并流行。鉴于 BVDV 存在多个亚基因型，在马来西亚边境对所有入境牛只进行筛查对于防止其他 BVDV 亚基因型进入该国非常重要。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文求助全文

来源期刊

Accounts of Chemical Research 化学-化学综合

CiteScore

31.40

自引率

1.10%

发文量

312

审稿时长

2 months

期刊介绍： Accounts of Chemical Research presents short, concise and critical articles offering easy-to-read overviews of basic research and applications in all areas of chemistry and biochemistry. These short reviews focus on research from the author’s own laboratory and are designed to teach the reader about a research project. In addition, Accounts of Chemical Research publishes commentaries that give an informed opinion on a current research problem. Special Issues online are devoted to a single topic of unusual activity and significance. Accounts of Chemical Research replaces the traditional article abstract with an article "Conspectus." These entries synopsize the research affording the reader a closer look at the content and significance of an article. Through this provision of a more detailed description of the article contents, the Conspectus enhances the article's discoverability by search engines and the exposure for the research.