Characterization of infectious laryngotracheitis virus isolated from commercial layer chickens in Bangladesh during the year 2021-2022.

IF 16.4 1区化学 Q1 CHEMISTRY, MULTIDISCIPLINARY

Accounts of Chemical Research Pub Date : 2024-06-09 eCollection Date: 2024-06-01 DOI:10.5455/javar.2024.k789

Md Mostofa Kamal, Mohammad Sadekuzzaman, Kohinoor Parvin, Md Enamul Haque, Sajedul Hayat, Md Ariful Islam, Mst Minara Khatun, Mahbubul Pratik Siddique, Sham Soun Nahar, A K M Khasruzzaman, Muhammud Tofazzal Hossain, Md Alimul Islam

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引用次数: 0

Abstract

Objective: Infectious laryngotracheitis virus (ILTV) is responsible for causing infectious laryngotracheitis (ILT), which is a rapidly spreading and extremely transmissible disease in chickens. The current research aims to isolate and characterize ILTV from layer chickens in Bangladesh.

Materials and methods: A total of 345 samples (trachea, larynx, and lungs) were collected from ILT-suspected dead and sick layer chickens of 32 ILT-suspected farms in three different outbreak districts (Gazipur, Tangail, and Mymensingh) of Bangladesh during the outbreak year 2021-2022. Rapid detection kits examined the samples for avian influenza virus (AIV) and Newcastle disease virus (NDV). ILTV-specific primers were used to screen 72 NDV- and AIV-negative samples by polymerase chain reaction (PCR). Using chorioallantoic membrane (CAM), the study isolated the ILT virus from 9 to 10-day-old seronegative embryonated chicken eggs (ECEs) using selected PCR-positive samples. The virus was confirmed using nucleotide sequencing, agar gel immunodiffusion test (AGIDT), viral neutralization test (VNT), and pathogenicity evaluations using mortality index for chicken embryos (MICEs) and intra-tracheal pathogenicity index (ITPI).

Results: The results indicated that among the PCR-positive 10 samples, only two (Alim_ILT_1001 and Alim_ILT_1,000) were found positive using ECEs. There were two field isolates of ILTVs, as shown by the amplicon size of the ICP4 gene-based PCR. A phylogenetic study of the ICP4 gene revealed that the recent isolates have a close similarity with the ILTV isolates of Turkey, Bangladesh, and Australia. AGIDT revealed strong precipitation lines due to ILTV-specific antibodies reacting with field viruses, while VNT neutralized both isolates with conventional ILTV antibodies. The pathogenicity testing indicated that Alim_ILT_1001 had MICE and ITPI values of 0.77 and 0.63, whereas Alim_ILT_1,000 had 0.71 and 0.57.

Conclusion: Both the ILTV isolates have similarities with the isolates of Turkey, Bangladesh, and Australia, and they are highly virulent for chickens.

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2021-2022 年孟加拉国商品蛋鸡中分离出的传染性喉气管炎病毒的特征。

目的：传染性喉气管炎病毒（ILTV传染性喉气管炎病毒（ILTV）是引起传染性喉气管炎（ILT）的罪魁祸首，该病在鸡中传播迅速，传染性极强。目前的研究旨在从孟加拉国的蛋鸡中分离并鉴定 ILTV：在 2021-2022 年疫情爆发年期间，从孟加拉国三个不同疫区（加济布尔、坦盖尔和迈门辛）32 个疑似 ILT 鸡场的疑似 ILT 死亡和患病蛋鸡身上共收集了 345 份样本（气管、喉咙和肺部）。快速检测试剂盒对样本进行了禽流感病毒（AIV）和新城疫病毒（NDV）检测。使用 ILTV 特异性引物通过聚合酶链式反应 (PCR) 筛选 72 份 NDV 和 AIV 阴性样本。该研究利用绒毛膜（CAM），从 9 至 10 日龄血清反应阴性的胚胎鸡卵（ECE）中分离出 ILT 病毒。通过核苷酸测序、琼脂凝胶免疫扩散试验（AGIDT）、病毒中和试验（VNT）以及鸡胚死亡率指数（MICE）和气管内致病性指数（ITPI）进行致病性评估，对病毒进行了确认：结果表明，在 PCR 阳性的 10 个样本中，只有两个样本（Alim_ILT_1001 和 Alim_ILT_1,000）通过 ECEs 发现呈阳性。从基于 ICP4 基因的 PCR 扩增子大小来看，ILTVs 有两个野外分离株。对 ICP4 基因的系统发育研究表明，最近分离的病毒与土耳其、孟加拉国和澳大利亚分离的 ILTV 病毒非常相似。AGIDT显示了因ILTV特异性抗体与野外病毒反应而产生的强沉淀线，而VNT则用传统的ILTV抗体中和了这两个分离株。致病性测试表明，Alim_ILT_1001 的 MICE 值和 ITPI 值分别为 0.77 和 0.63，而 Alim_ILT_1,000 的 MICE 值和 ITPI 值分别为 0.71 和 0.57：这两种 ILTV 分离物与土耳其、孟加拉国和澳大利亚的分离物有相似之处，对鸡具有很强的毒性。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Accounts of Chemical Research 化学-化学综合

CiteScore

31.40

自引率

1.10%

发文量

312

审稿时长

2 months

期刊介绍： Accounts of Chemical Research presents short, concise and critical articles offering easy-to-read overviews of basic research and applications in all areas of chemistry and biochemistry. These short reviews focus on research from the author’s own laboratory and are designed to teach the reader about a research project. In addition, Accounts of Chemical Research publishes commentaries that give an informed opinion on a current research problem. Special Issues online are devoted to a single topic of unusual activity and significance. Accounts of Chemical Research replaces the traditional article abstract with an article "Conspectus." These entries synopsize the research affording the reader a closer look at the content and significance of an article. Through this provision of a more detailed description of the article contents, the Conspectus enhances the article's discoverability by search engines and the exposure for the research.