Autologous Serum Eye Drops Diluted with Cyclosporine A 0.05% and Sodium Hyaluronate 0.1%: An Experimental Comparative Study.

IF 1.7 4区医学 Q3 OPHTHALMOLOGY

Current Eye Research Pub Date : 2024-08-04 DOI:10.1080/02713683.2024.2385442

Rajan Sharma, Ashok Sharma, Vandita Kakkar, Komal Saini, Janardhana P Balakrishna, Verinder S Nirankari

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引用次数: 0

Abstract

Purpose: The purpose of this study was to assess in-vitro efficacy of a suffusion of autologous serum withcyclosporine 0.05% (CsA) and sodium hyaluronate 0.1% (SH).

Methods: The expression of proinflammatory markers interleukin 6 (IL-6) and TNF-Alpha (TNF-α) in limbal epithelial cells was evaluated. Also, assessment of the stability of epithelial growth factor and transforming growth factor-beta (EGF, TGF-β) in the 50% combinations with autologous serum (AS) was done. The characteristics (pH, density, osmolality) of the two combinations were also evaluated. Additionally, cytotoxicity effect of given test compounds was evaluated on human limbal epithelial cells (LEpiC).

Results: The percentage of cells expressing IL-6 subjected to AS + SH and AS + CsA were 6.23% and 5.69% respectively. There was no significant difference in percentage of cells expressing TNF-α between the formulations (5.87%, 5.83% respectively). The growth factors; EGF and TGF-β remained stable forone month duration (on 2 and 4 weeks) at 4 °C without significant difference between the time intervals tested. The results of MTT assay suggested that limbal epithelial cells treated with AS + CsA and AS + SH combinations showed minimal toxicity however it was not significant statistically (p ≤ 0.05).

Conclusion: Two test combinations (AS + CsA, AS + SH) showed stable growth factors (EGF, TGF-β) and good anti-inflammatory property against pro-inflammatory markers. Also, the 2 combinations were found safe on cultured limbal epithelial cells. The novel combination of autologous serum in CsA may provide added benefit in dry eye disease (DED) through their combined anti-inflammatory and epitheliotropic effects.

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用环孢素 A 0.05% 和透明质酸钠 0.1% 稀释的自体血清滴眼液：实验对比研究。

目的：本研究旨在评估自体血清与 0.05% 环孢素（CsA）和 0.1% 透明质酸钠（SH）混合液的体外疗效：方法：评估了白细胞介素 6（IL-6）和 TNF-α（TNF-α）这两种促炎标志物在角膜缘上皮细胞中的表达。此外，还评估了上皮生长因子和转化生长因子-β（EGF、TGF-β）在与自体血清（AS）50%的组合中的稳定性。同时还评估了两种组合的特性（pH 值、密度、渗透压）。此外，还评估了给定测试化合物对人眼睑上皮细胞（LEpiC）的细胞毒性效应：结果：AS + SH 和 AS + CsA 的细胞表达 IL-6 的百分比分别为 6.23% 和 5.69%。两种制剂中表达 TNF-α 的细胞比例差异不大（分别为 5.87%和 5.83%）。生长因子 EGF 和 TGF-β 在 4 °C、一个月的时间内（2 周和 4 周）保持稳定，测试时间间隔之间没有明显差异。MTT 检测结果表明，用 AS + CsA 和 AS + SH 组合处理的睑缘上皮细胞毒性很小，但统计学意义不显著（P ≤ 0.05）：两种试验组合（AS + CsA、AS + SH）显示出稳定的生长因子（EGF、TGF-β）和良好的抗炎特性，可对抗促炎标志物。此外，这两种组合对培养的睑缘上皮细胞也是安全的。自体血清与 CsA 的新型组合可通过其联合抗炎和上皮细胞促进作用为干眼症（DED）带来更多益处。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Current Eye Research 医学-眼科学

CiteScore

4.60

自引率

0.00%

发文量

163

审稿时长

12 months

期刊介绍： The principal aim of Current Eye Research is to provide rapid publication of full papers, short communications and mini-reviews, all high quality. Current Eye Research publishes articles encompassing all the areas of eye research. Subject areas include the following: clinical research, anatomy, physiology, biophysics, biochemistry, pharmacology, developmental biology, microbiology and immunology.