CD34+ stromal cells/telocytes and their role in mouse lung development: Light microscopy, immunofluorescence, ultrastructural and scanning electron microscopy evidence

IF 4.3 3区 材料科学 Q1 ENGINEERING, ELECTRICAL & ELECTRONIC
Ganesh Dama, Chengxu Xue, Yangxia Zhang, Dezhuang Li, Jinyu Fan, Liang Qiao, Zhihao Xu, Ciqing Yang, Yanli Liu, Mohammad Farris Iman Leong Bin Abdullah, Juntang Lin
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Abstract

Telocytes (TCs), a novel type of mesenchymal or interstitial cell with specific, very long and thin cellular prolongations, have been found in various mammalian organs and have potential biological functions. However, their existence during lung development is poorly understood. This study aimed to investigate the existence, morphological features, and role of CD34+ SCs/TCs in mouse lungs from foetal to postnatal life using primary cell culture, double immunofluorescence, transmission electron microscopy (TEM) and scanning electron microscopy (SEM). The immunofluorescence double staining profiles revealed positive expression of CD34 and PDGFR-α, Sca-1 or VEGFR-3, and the expression of these markers differed among the age groups during lung development. Intriguingly, in the E18.5 stage of development, along with the CD34+ SCs/TCs, haematopoietic stem cells and angiogenic factors were also significantly increased in number compared with those in the E14.5, E16.5, P0 and P7. Subsequently, TEM confirmed that CD34+ SCs/TCs consisted of a small cell body with long telopodes (Tps) that projected from the cytoplasm. Tps consisted of alternating thin and thick segments known as podomers and podoms. TCs contain abundant endoplasmic reticulum, mitochondria and secretory vesicles and establish close connections with neighbouring cells. Furthermore, SEM revealed characteristic features, including triangular, oval, spherical, or fusiform cell bodies with extensive cellular prolongations, depending on the number of Tps. Our findings provide evidence for the existence of CD34+ SCs/TCs, which contribute to vasculogenesis, the formation of the air‒blood barrier, tissue organization during lung development and homoeostasis.

CD34+ 基质细胞/骨髓细胞及其在小鼠肺发育中的作用:光镜、免疫荧光、超微结构和扫描电子显微镜证据。
远端细胞(TCs)是一种新型间质细胞或间质细胞,具有特异性的、非常细长的细胞延伸,已在多种哺乳动物器官中发现,并具有潜在的生物学功能。然而,人们对它们在肺发育过程中的存在还知之甚少。本研究旨在采用原代细胞培养、双重免疫荧光、透射电子显微镜(TEM)和扫描电子显微镜(SEM)等方法,研究CD34+ SCs/TCs在小鼠从胎儿到出生后肺部的存在、形态特征和作用。免疫荧光双重染色图谱显示,CD34和PDGFR-α、Sca-1或VEGFR-3呈阳性表达,这些标记物在肺发育过程中的表达在不同年龄组间存在差异。耐人寻味的是,与E14.5、E16.5、P0和P7相比,在E18.5发育阶段,除了CD34+ SCs/TCs,造血干细胞和血管生成因子的数量也显著增加。随后,TEM 证实,CD34+ SCs/TCs 由一个小细胞体和从细胞质中伸出的长端粒(Tps)组成。Tps由被称为podomers和podoms的薄片和厚片交替组成。TC含有丰富的内质网、线粒体和分泌泡,并与邻近细胞建立密切联系。此外,扫描电子显微镜(SEM)显示了一些特征,包括三角形、椭圆形、球形或纺锤形细胞体,这些细胞体具有广泛的细胞延伸,具体取决于 Tps 的数量。我们的研究结果为 CD34+ SCs/TCs 的存在提供了证据,它们有助于血管生成、气血屏障的形成、肺部发育过程中的组织结构和平衡。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
CiteScore
7.20
自引率
4.30%
发文量
567
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