A comprehensive review on fructosyl peptide oxidase as an important enzyme for present hemoglobin A1c assays.

IF 3.2 4区生物学 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY

Biotechnology and applied biochemistry Pub Date : 2024-08-04 DOI:10.1002/bab.2647

Seyyed Soheil Rahmatabadi, Hoda Bashiri, Bijan Soleymani

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Abstract

Glycated proteins are generated by binding of glucose to the proteins in blood stream through a nonenzymatic reaction. Hemoglobin A1c (HbA1c) is a glycated protein with glucose at the N-terminal of β-chain. HbA1c is extensively used as an indicator for assessing the blood glucose concentration in diabetes patients. There are different conventional clinical methods for the detection of HbA1c. However, enzymatic detection method has newly obtained great attention for its high precision and cost-effectiveness. Today, fructosyl peptide oxidase (FPOX) plays a key role in the enzymatic measurement of HbA1c, and different companies have marketed HbA1c assay systems based on FPOX. Recent investigations show that FPOX could be used in assaying HbA1 without requiring HbA1c primary digestion. It could also be applied as a biosensor for HbA1c detection. In this review, we have discussed the recent improvements of FPOX properties, different methods of FPOX purification, solubility, and immobilization, and also the use of FPOX in HbA1c biosensors.

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关于果糖肽氧化酶的全面综述，它是目前血红蛋白 A1c 检测中的一种重要酶。

糖化蛋白质是葡萄糖通过非酶反应与血流中的蛋白质结合而生成的。血红蛋白 A1c（HbA1c）是一种糖化蛋白质，其 β 链的 N 端含有葡萄糖。HbA1c 被广泛用作评估糖尿病患者血糖浓度的指标。临床上有不同的传统方法来检测 HbA1c。然而，酶法检测因其精确度高、成本效益高而备受关注。目前，果糖基肽氧化酶（FPOX）在 HbA1c 的酶法检测中发挥着关键作用，不同的公司已经推出了基于 FPOX 的 HbA1c 检测系统。最近的研究表明，FPOX 可用于检测 HbA1，而无需对 HbA1c 进行初级消化。它还可用作检测 HbA1c 的生物传感器。在这篇综述中，我们讨论了 FPOX 特性的最新改进，FPOX 纯化、溶解和固定的不同方法，以及 FPOX 在 HbA1c 生物传感器中的应用。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Biotechnology and applied biochemistry 工程技术-生化与分子生物学

CiteScore

6.00

自引率

7.10%

发文量

117

审稿时长

3 months

期刊介绍： Published since 1979, Biotechnology and Applied Biochemistry is dedicated to the rapid publication of high quality, significant research at the interface between life sciences and their technological exploitation. The Editors will consider papers for publication based on their novelty and impact as well as their contribution to the advancement of medical biotechnology and industrial biotechnology, covering cutting-edge research in synthetic biology, systems biology, metabolic engineering, bioengineering, biomaterials, biosensing, and nano-biotechnology.