A GC-MS Metabolic Study on Lipophilic Compounds in the Leaves of Common Wheat Triticum aestivum L.

IF 3.4 3区生物学 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY

Metabolites Pub Date : 2024-08-01 DOI:10.3390/metabo14080426

Asya R. Vasilieva, Nikolay M. Slynko, Nikolay P. Goncharov, Ljudmila E. Tatarova, Leonid V. Kuibida, Sergey E. Peltek

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Abstract

Common wheat (Triticum aestivum L.) is one of the most valuable cereal crops worldwide. This study examined leaf extracts of 30 accessions of T. aestivum and its subspecies using 48 h maceration with methanol by GC-MS and GCxGC-MS. The plants were grown from seeds of the wheat genetics collection of the Wheat Genetics Sector of the Institute of Cytology and Genetics, SB RAS. The analysis revealed 263 components of epicuticular waxes, including linear and branched alkanes, aliphatic alcohols, aldehydes, ketones, β-diketones, carboxylic acids and their derivatives, mono- and diterpenes, phytosterols, and tocopherols. Hierarchical cluster analysis and principal component analysis were used to identify and visualize the differences between the leaf extracts of different wheat cultivars. Three clusters were identified, with the leading components being (1) octacosan-1-ol, (2) esters of saturated and unsaturated alcohols, and (3) fatty acid alkylamides, which were found for the first time in plant extracts. The results highlight the importance of metabolic studies in understanding the adaptive mechanisms and increasing wheat resistance to stress factors. These are crucial for breeding new-generation cultivars with improved traits.

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普通小麦叶片中亲脂性化合物的气相色谱-质谱代谢研究

普通小麦（Triticum aestivum L.）是全球最有价值的谷类作物之一。本研究采用 GC-MS 和 GCxGC-MS 方法，研究了用甲醇浸泡 48 小时的 30 种 T. aestivum 及其亚种的叶提取物。这些植株是从 SB RAS 细胞学和遗传学研究所小麦遗传学部门的小麦遗传学收藏品种子中培育出来的。分析结果显示，表皮蜡质中有 263 种成分，包括直链和支链烷烃、脂肪醇、醛、酮、β-二酮、羧酸及其衍生物、单萜和二萜、植物甾醇和生育酚。采用层次聚类分析和主成分分析来确定和显示不同小麦栽培品种叶提取物之间的差异。结果发现了三个聚类，其主要成分为：(1) 辛可散-1-醇；(2) 饱和及不饱和醇酯；(3) 脂肪酸烷基酰胺，这是首次在植物提取物中发现脂肪酸烷基酰胺。这些结果突显了代谢研究在了解适应机制和提高小麦抗逆性方面的重要性。这些对于培育具有改良性状的新一代栽培品种至关重要。

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来源期刊

Metabolites Biochemistry, Genetics and Molecular Biology-Molecular Biology

CiteScore

5.70

自引率

7.30%

发文量

1070

审稿时长

17.17 days

期刊介绍： Metabolites (ISSN 2218-1989) is an international, peer-reviewed open access journal of metabolism and metabolomics. Metabolites publishes original research articles and review articles in all molecular aspects of metabolism relevant to the fields of metabolomics, metabolic biochemistry, computational and systems biology, biotechnology and medicine, with a particular focus on the biological roles of metabolites and small molecule biomarkers. Metabolites encourages scientists to publish their experimental and theoretical results in as much detail as possible. Therefore, there is no restriction on article length. Sufficient experimental details must be provided to enable the results to be accurately reproduced. Electronic material representing additional figures, materials and methods explanation, or supporting results and evidence can be submitted with the main manuscript as supplementary material.