Sensitive and selective detection of glycine betaine using curcumin-based fluorescent molecule with real sample analysis

IF 2.2 4区化学 Q3 CHEMISTRY, MULTIDISCIPLINARY

Journal of the Iranian Chemical Society Pub Date : 2024-08-03 DOI:10.1007/s13738-024-03071-1

Lavanya Raja, Sangeethapriya Balamuthu, Srinivasadesikan Venkatesan, Lin Ming-chang, Padmini Vediappen

引用次数: 0

Abstract

A highly selective biosensor for glycine betaine (GB) based on the curcumin derivative (1BHD) is described, which displays a significant visual change and a lower detection limit (76 nM). The GB sensor displays a 1:1 binding ratio and a binding constant of 9.042 × 10⁷ M⁻¹. The binding mechanism of 1BHD for glycine betaine was confirmed by ¹H NMR titration, mass spectrometry, and DFT calculation. The fluorescence intensity of 1BHD was selectively quenched by GB, and an obvious color change from yellow to orange was observed by the naked eye. The above results have been utilized to detect GB sensitively in blood and urine samples.

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利用基于姜黄素的荧光分子和真实样品分析，灵敏、选择性地检测甘氨酸甜菜碱

本文描述了一种基于姜黄素衍生物（1BHD）的高选择性甘氨酸甜菜碱（GB）生物传感器，它具有显著的视觉变化和较低的检测限（76 nM）。该 GB 传感器的结合率为 1:1，结合常数为 9.042 × 107 M-1。1BHD 与甘氨酸甜菜碱的结合机制通过 1H NMR 滴定、质谱分析和 DFT 计算得到了证实。GB 选择性地淬灭了 1BHD 的荧光强度，肉眼观察到其颜色由黄色明显变为橙色。上述结果已被用于在血液和尿液样品中灵敏地检测 GB。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Journal of the Iranian Chemical Society 化学-化学综合

CiteScore

4.40

自引率

8.30%

发文量

230

审稿时长

5.6 months

期刊介绍： JICS is an international journal covering general fields of chemistry. JICS welcomes high quality original papers in English dealing with experimental, theoretical and applied research related to all branches of chemistry. These include the fields of analytical, inorganic, organic and physical chemistry as well as the chemical biology area. Review articles discussing specific areas of chemistry of current chemical or biological importance are also published. JICS ensures visibility of your research results to a worldwide audience in science. You are kindly invited to submit your manuscript to the Editor-in-Chief or Regional Editor. All contributions in the form of original papers or short communications will be peer reviewed and published free of charge after acceptance.