Electrochemical detection of tumor cells based on proximity labelling-assisted multiple signal amplification†

IF 3.5 Q2 CHEMISTRY, ANALYTICAL
Guozhang Zhou, Fei Zhou, Xiaomeng Yu, Daiyuan Zhou, Jiaqi Wang, Bing Bo, Ya Cao and Jing Zhao
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引用次数: 0

Abstract

Malignant tumors are the second leading cause of human deaths worldwide, and early cancer screening and diagnosis can effectively reduce cancer mortality. Herein, we propose a new electrochemical method for the highly sensitive detection of MUC1-positive tumor cells based on proximity labelling-assisted multiple signal amplification. Specifically, a MUC1 aptamer-modified electrode was prepared for capturing MUC1-positive tumor cells, followed by binding of G4-DNA strands to the cells with the aid of a mild reduction reaction. A hemin/G4-DNA complex was then formed and acted as a mimic of horseradish peroxidase, catalysing the proximal labelling of tyramine-modified gold nanoparticles to induce silver-enhanced electrochemical signal amplification. Electrochemical results demonstrated that the method was able to specially identify MUC1-positive tumor cells and generate corresponding electrochemical responses in the range of 100 cells per mL to 1 × 106 cells per mL with a detection limit of 21 cells per mL. Furthermore, the method displayed good stability and anti-interference performance in complex serum environments. Therefore, our work may provide an effective tool to improve the accuracy of cell-based tissue examination and liquid biopsy for early diagnosis of cancers in the future.

Abstract Image

基于近距离标记辅助多重信号放大技术的肿瘤细胞电化学检测技术
恶性肿瘤是导致全球人类死亡的第二大原因,早期癌症筛查和诊断可有效降低癌症死亡率。在此,我们提出了一种基于近距离标记辅助多重信号放大的高灵敏度检测 MUC1 阳性肿瘤细胞的新型电化学方法。具体来说,我们制备了用于捕获 MUC1 阳性肿瘤细胞的 MUC1 合物修饰电极,然后借助温和的还原反应将 G4-DNA 链与细胞结合。然后形成血红素/G4-DNA 复合物,作为辣根过氧化物酶的模拟物,催化酪胺修饰金纳米粒子的近端标记,诱导银增强电化学信号放大。电化学结果表明,该方法能专门识别 MUC1 阳性肿瘤细胞,并在 100 个细胞/毫升至 1×106 个细胞/毫升的范围内产生相应的电化学反应,检测限为 21 个细胞/毫升。此外,该方法在复杂的血清环境中显示出良好的稳定性和抗干扰性能。因此,我们的工作可为提高基于细胞的组织检查和液体活检的准确性提供有效工具,从而在未来实现癌症的早期诊断。
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CiteScore
2.30
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