A combination of physiology, metabolomics, and genetics reveals the two-component system ResS/ResR-mediated Fe and Al release from biotite by Pseudomonas pergaminensis F77

IF 6.1 1区 生物学 Q1 MICROBIOLOGY
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Abstract

Understanding of the mechanisms on bacteria-regulated mineral dissolution functions is important for further insight into mineral-microbe interactions. The functions of the two-component system have been studied. However, the molecular mechanisms involved in bacterial two-component system-mediated mineral dissolution are poorly understood. Here, the two-component regulatory system ResS/ResR in the mineral-solubilizing bacterium Pseudomonas pergaminensis F77 was characterized for its involvement in biotite dissolution. Strain F77 and the F77ΔresS, F77ΔresR, and F77ΔresS/R mutants were constructed and compared for the ResS/ResR system-mediated Fe and Al release from biotite in the medium and the mechanisms involved. After 3 days of incubation, the F77ΔresS, F77ΔresR, and F77ΔresS/R mutants significantly decreased the Fe and Al concentrations in the medium compared with F77. The F77ΔresS/R mutant had a greater impact on Fe and Al release from biotite than did the F77ΔresS or F77ΔresR mutant. The F77∆resS/R mutant exhibited significantly reduced Fe and Al concentrations by 21–61 % between 12 h and 48 h of incubation compared with F77. Significantly increased pH values and decreased cell counts on the mineral surfaces were found in the presence of the F77∆resS/R mutant compared with those in the presence of F77 between 12 h and 48 h of incubation. Metabolomic analysis revealed that the extracellular metabolites associated with biotite dissolution were downregulated in the F77ΔresS/R mutant. These downregulated metabolites included GDP-fucose, 20-carboxyleukotriene B4, PGP (16:1(9Z)/16:0), 3′,5′-cyclic AMP, and a variety of acidic metabolites involved in carbohydrate, amino acid, and lipid metabolisms, glycan biosynthesis, and cellular community function. Furthermore, the expression levels of the genes involved in the production of these metabolites were downregulated in the F77ΔresS/R mutant compared with those in F77. Our findings suggested that the ResS/ResR system in F77 contributed to mineral dissolution by mediating the production of mineral-solubilizing related extracellular metabolites and bacterial adsorption on mineral surface.

生理学、代谢组学和遗传学的结合揭示了由假单胞菌 Pergaminensis F77 介导的双组分系统 ResS/ResR 从生物岩中释放铁和铝。
了解细菌调节矿物溶解功能的机制对于进一步了解矿物与微生物之间的相互作用非常重要。人们已经对双组分系统的功能进行了研究。然而,人们对细菌双组分系统介导矿物溶解的分子机制知之甚少。在此,研究人员对矿物溶解假单胞菌 F77 中的双组分调控系统 ResS/ResR 进行了表征,以确定其在矿物溶解过程中的参与情况。研究人员构建了菌株 F77 和 F77ΔresS、F77ΔresR 和 F77ΔresS/R 突变体,并比较了 ResS/ResR 系统介导的培养基中生物岩铁和铝的释放及其机制。培养 3 天后,与 F77 相比,F77ΔresS、F77ΔresR 和 F77ΔresS/R 突变体显著降低了培养基中铁和铝的浓度。与 F77ΔresS 或 F77ΔresR 突变体相比,F77ΔresS/R 突变体对生物铁矿石中铁和铝释放的影响更大。与 F77 相比,F77ΔresS/R 突变体在培养 12 小时至 48 小时期间的铁和铝浓度明显降低了 21-61%。与 F77 相比,F77ΔresS/R 突变体存在时,矿物表面的 pH 值明显升高,细胞数量减少。代谢组分析表明,在 F77ΔresS/R 突变体中,与生物岩溶解有关的细胞外代谢物被下调。这些下调的代谢物包括 GDP-岩藻糖、20-羧基白三烯 B4、PGP (16:1(9Z)/16:0)、3',5'-环 AMP,以及涉及碳水化合物、氨基酸和脂质代谢、糖类生物合成和细胞群落功能的多种酸性代谢物。此外,与 F77 相比,参与产生这些代谢物的基因在 F77ΔresS/R 突变体中的表达水平下调。我们的研究结果表明,F77中的ResS/ResR系统通过介导矿物溶解相关胞外代谢物的产生和细菌在矿物表面的吸附,促进了矿物的溶解。
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来源期刊
Microbiological research
Microbiological research 生物-微生物学
CiteScore
10.90
自引率
6.00%
发文量
249
审稿时长
29 days
期刊介绍: Microbiological Research is devoted to publishing reports on prokaryotic and eukaryotic microorganisms such as yeasts, fungi, bacteria, archaea, and protozoa. Research on interactions between pathogenic microorganisms and their environment or hosts are also covered.
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