Chondrocyte Response to Fresh Autologous Conditioned Serum Versus Freeze-Dried Allogenic Conditioned Serum.

IF 2.7 4区医学 Q1 ORTHOPEDICS

CARTILAGE Pub Date : 2024-08-02 DOI:10.1177/19476035241261335

Kevin Credille, Tristan J Elias, Sachin Allahabadi, Zachary Wang, Arnavaz Hakimiyan, Susan Chubinskaya, Brian J Cole, David Frisbie, Adam B Yanke

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引用次数: 0

Abstract

Objective: To investigate the cytokine release profile and histological response of human cartilage after exposure to autologous conditioned serum (ACS) and freeze-dried allogenic conditioned serum (FD-CS).

Design: Cartilage explants were collected from 6 patients undergoing total knee arthroplasty. ACS and FD-CS were created from patient serum samples. Cartilage samples were divided into 6 groups: (1) untreated control, (2) ACS, (3) FD-CS, (4) untreated interleukin (IL)-1β (5 ng/ml), (5) IL-1β + ACS, and (6) IL-1β + FD-CS. After 12 days, cartilage samples were analyzed with glycosaminoglycan (GAG) concentration normalized to wet weight while comparing cytokine concentrations, and histological scoring.

Results: There was a significant decrease in pathology scoring for ACS (P = 0.0368) and FD-CS (P = 0.0368) in the IL-1β injury groups compared with the untreated IL-1β insult group. ACS and FD-CS significantly mitigate the IL-1β induced increase in basic fibroblast growth factor (bFGF) (P = 0.0009 and P = 0.0002, respectively). FD-CS showed a significant decrease in IL-1β concentration in the presence of IL-1β insult compared with the untreated IL-1β group (P < 0.0001). ACS-treated samples had significantly higher concentration of tumor necrosis factor (TNF)-α independent of IL-1β when compared with samples not treated with biologics (P = 0.0053).

Conclusions: Explanted osteoarthritic cartilage responds favorably and equivalently to treatment with ACS and FD-CS from a histological perspective. Both ACS and FD-CS were able to mitigate the IL-1β-induced increases in bFGF and FD-CS lowered IL-1β concentration while increasing interleukin-1 receptor antagonist (IL-1Ra) concentration. Although the cytokine profile of cartilage tissue explants treated with FD-CS appears to be different than that of ACS, this difference does not seem to affect biologic activity of FD-CS.

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软骨细胞对新鲜自体调节血清和冻干异源调节血清的反应

目的研究暴露于自体调节血清（ACS）和冻干异体调节血清（FD-CS）后人体软骨的细胞因子释放谱和组织学反应：设计：从 6 名接受全膝关节置换术的患者身上采集软骨外植体。从患者血清样本中提取 ACS 和 FD-CS。软骨样本分为 6 组：(1) 未经处理的对照组；(2) ACS；(3) FD-CS；(4) 未经处理的白细胞介素 (IL)-1β (5 ng/ml)；(5) IL-1β + ACS；(6) IL-1β + FD-CS。12 天后，对软骨样本进行分析，糖胺聚糖（GAG）浓度与湿重标准化，同时比较细胞因子浓度和组织学评分：结果：与未处理的 IL-1β 损伤组相比，IL-1β 损伤组的 ACS（P = 0.0368）和 FD-CS （P = 0.0368）病理评分明显下降。ACS 和 FD-CS 能显著缓解 IL-1β 诱导的碱性成纤维细胞生长因子（bFGF）的增加（分别为 P = 0.0009 和 P = 0.0002）。与未处理的 IL-1β 组相比，FD-CS 在 IL-1β 侮辱的情况下显示出 IL-1β 浓度的显著下降（P < 0.0001）。与未经生物制剂处理的样本相比，经 ACS 处理的样本中肿瘤坏死因子（TNF）-α 的浓度明显更高，与 IL-1β 无关（P = 0.0053）：结论：从组织学角度看，骨关节炎软骨移植后对 ACS 和 FD-CS 治疗的反应良好，且效果相当。ACS和FD-CS都能缓解IL-1β诱导的bFGF的增加，FD-CS能降低IL-1β的浓度，同时增加白细胞介素-1受体拮抗剂（IL-1Ra）的浓度。虽然经 FD-CS 处理的软骨组织外植体的细胞因子谱似乎与 ACS 不同，但这种差异似乎不会影响 FD-CS 的生物活性。

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来源期刊

CARTILAGE ORTHOPEDICS-

CiteScore

6.90

自引率

7.10%

发文量

期刊介绍： CARTILAGE publishes articles related to the musculoskeletal system with particular attention to cartilage repair, development, function, degeneration, transplantation, and rehabilitation. The journal is a forum for the exchange of ideas for the many types of researchers and clinicians involved in cartilage biology and repair. A primary objective of CARTILAGE is to foster the cross-fertilization of the findings between clinical and basic sciences throughout the various disciplines involved in cartilage repair. The journal publishes full length original manuscripts on all types of cartilage including articular, nasal, auricular, tracheal/bronchial, and intervertebral disc fibrocartilage. Manuscripts on clinical and laboratory research are welcome. Review articles, editorials, and letters are also encouraged. The ICRS envisages CARTILAGE as a forum for the exchange of knowledge among clinicians, scientists, patients, and researchers. The International Cartilage Repair Society (ICRS) is dedicated to promotion, encouragement, and distribution of fundamental and applied research of cartilage in order to permit a better knowledge of function and dysfunction of articular cartilage and its repair.