Impact of low-to moderate-intensity exercise training on the mRNA expression of purine receptors across various vessels in SHR.

Yue Ma, Jin Du, Xin-Xin Wang, Tong Deng, Jia-Li Qi, Hong Cheng, Lu Li
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Abstract

Objective: In this study, we developed an exercise training protocol for assessing both blood pressure dynamics and mRNA expression levels of purine receptors in various vascular tissues during physical activity. The objective is to assess the impact of exercise training on blood pressure regulation in spontaneously hypertensive rats (SHR) and purine receptors in vascular tissues.

Methods: Wistar Kyoto (WKY) and SHR rats were randomly allocated into sedentary (Sed) and exercise training (ExT) groups. Rats in the Sed groups were allowed unrestricted movement, whereas those in the ExT groups underwent a 16-week regimen of low- to moderate-intensity treadmill exercise. Throughout the intervention period, blood pressure measurements and body weight recordings were conducted. Additionally, mRNA expressions of purine receptors P2X1, P2Y1, and P2Y2 in renal artery (RA), internal carotid artery (Int), thoracic aorta (Aor), and caudal artery (Cau) tissues were assessed.

Results: In the Sed group, body weight of SHR rats was observed to be lower compared to the three other groups. Over the course of the exercise regimen, blood pressure in the ExT group of SHR rats reduced gradually, converging towards levels similar to those observed in WKY rats by the conclusion of the exercise period. Regarding mRNA expression patterns of P2X1 receptors across the four blood vessels, WKY and SHR rats demonstrated similar sequences, consistently displaying the highest expression levels in the Cau. Conversely, mRNA expressions of P2Y1 and P2Y2 receptors exhibited distinct sequences across the four blood vessels in both WKY and SHR rats. Notably, compared to the Sed group of WKY rats, mRNA expression of P2X1 receptor in the Int of SHR rats revealed an increase, while expressions in the Aor of WKY rats and the Cau of SHR rats decreased following exercise. Expression of P2Y1 receptor mRNA decreased across all four types of blood vessels in SHR rats. Post-exercise, P2Y1 receptor mRNA expression increased in the Aor, decreased in the Cau of WKY rats, and increased in the Int and renal artery (RA) of SHR rats. Conversely, expressions of P2Y2 receptor mRNA decreased in the Int and Aor of SHR rats. Except for the Aor of WKY rats, expressions of P2Y2 receptor mRNA increased in the other arteries of both rat types following exercise.

Conclusion: Differences in the distribution of purine receptor subtypes among distinct arterial segments in both WKY and SHR rats were observed. Exercise training was found to enhance mRNA expression levels of P2Y receptors in these rat models. This finding implies that exercise training might reduce hypertension in SHR rats by bolstering the purinergic relaxation response.

中低强度运动训练对 SHR 不同血管中嘌呤受体 mRNA 表达的影响。
研究目的在这项研究中,我们制定了一种运动训练方案,用于评估体育活动期间的血压动态和各种血管组织中嘌呤受体的 mRNA 表达水平。目的是评估运动训练对自发性高血压大鼠(SHR)血压调节和血管组织中嘌呤受体的影响:方法:将 Wistar Kyoto(WKY)和 SHR 大鼠随机分为静坐组(Sed)和运动训练组(ExT)。静坐组大鼠的活动不受限制,而运动训练组大鼠则接受为期16周的中低强度跑步机运动。在整个干预期间,对大鼠进行血压测量和体重记录。此外,还评估了肾动脉(RA)、颈内动脉(Int)、胸主动脉(Aor)和尾动脉(Cau)组织中嘌呤受体 P2X1、P2Y1 和 P2Y2 的 mRNA 表达:结果:在Sed组,观察到SHR大鼠的体重低于其他三组。在运动过程中,ExT 组 SHR 大鼠的血压逐渐降低,在运动结束时趋于与 WKY 大鼠相似的水平。关于四种血管中 P2X1 受体 mRNA 的表达模式,WKY 和 SHR 大鼠表现出相似的序列,Cau 的表达水平始终最高。相反,在 WKY 和 SHR 大鼠的四条血管中,P2Y1 和 P2Y2 受体的 mRNA 表达表现出不同的序列。值得注意的是,与 Sed 组 WKY 大鼠相比,运动后 SHR 大鼠 Int 中 P2X1 受体 mRNA 表达量增加,而 WKY 大鼠 Aor 和 SHR 大鼠 Cau 中的表达量减少。在 SHR 大鼠的所有四种类型血管中,P2Y1 受体 mRNA 的表达均有所下降。运动后,P2Y1 受体 mRNA 在 WKY 大鼠的 Aor 中表达增加,在 Cau 中表达减少,而在 SHR 大鼠的 Int 和肾动脉 (RA) 中表达增加。相反,P2Y2 受体 mRNA 在 SHR 大鼠的 Int 和 Aor 中的表达量减少。除 WKY 大鼠的 Aor 外,两种大鼠的其他动脉在运动后 P2Y2 受体 mRNA 的表达均有所增加:结论:观察到嘌呤受体亚型在 WKY 和 SHR 大鼠不同动脉节段的分布存在差异。在这些大鼠模型中,运动训练可提高 P2Y 受体的 mRNA 表达水平。这一发现意味着,运动训练可通过增强嘌呤能松弛反应来降低 SHR 大鼠的高血压。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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