TNF reduces osteogenic cell fate in PDL cells at transcriptional and functional levels without alteration of periodontal proliferative capacity.

IF 1.3 4区 医学 Q3 DENTISTRY, ORAL SURGERY & MEDICINE
Isabel Knaup, Rafael Kramann, Martha-Julia Sasula, Paula Mack, Rogério Bastos Craveiro, Christian Niederau, Franziska Coenen, Sabine Neuss, Joachim Jankowski, Michael Wolf
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引用次数: 0

Abstract

Aims: To investigate the effect of tumor necrosis factor (TNF) on the growth of human periodontal ligament (PDL) cells, their osteogenic differentiation and modulation of their matrix secretion in vitro.

Methods: The influence of 10 ng/ml TNF on proliferation and metabolic activity of PDL cells was analyzed by cell counting (DAPI [4',6-diamidino-2-phenylindole] staining) and the MTS (3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium) assay. In addition, cells were cultured under control conditions and osteogenic conditions (media containing 10 mM β-glycerophosphate). Quantitative expression analysis of genes encoding the osteogenic markers alkaline phosphatase (ALP), collagen type I alpha 1 chain (COL1A1), osteoprotegerin (OPG), and osteopontin (OPN) was performed after 7 and 14 days of cultivation. Calcium deposits were stained with alizarin red.

Results: Our studies showed that 10 ng/ml TNF did not affect the survival and metabolic activity of PDL cells. Quantitative expression analysis revealed that long-term cultures with TNF impaired osteogenic cell fate at early and late developmental stages. Furthermore, TNF significantly reduced matrix secretion in PDL cells.

Conclusion: The present data confirm TNF as a regulatory factor of proinflammatory remodeling that influences the differentiation behavior but not the metabolism and cell proliferation of the periodontium. Therefore, TNF represents an interesting target for the regulation of orthodontic remodeling processes in the periodontium.

Abstract Image

TNF 可在转录和功能水平上减少 PDL 细胞的成骨细胞命运,但不会改变牙周增殖能力。
目的:研究肿瘤坏死因子(TNF)对体外人牙周韧带(PDL)细胞生长、成骨分化及其基质分泌的影响:方法:通过细胞计数(DAPI[4',6-二脒基-2-苯基吲哚]染色)和MTS(3-(4,5-二甲基噻唑-2-基)-5-(3-羧基甲氧基苯基)-2-(4-磺酸苯基)-2H-四氮唑)试验分析10 ng/ml TNF对PDL细胞增殖和代谢活性的影响。此外,还在对照条件和成骨条件(含 10 mM β-甘油磷酸酯的培养基)下培养细胞。培养 7 天和 14 天后,对编码成骨标志物碱性磷酸酶(ALP)、Ⅰ型胶原α1 链(COL1A1)、骨保护素(OPG)和骨生成素(OPN)的基因进行定量表达分析。钙沉积物用茜素红染色:我们的研究表明,10 ng/ml TNF 不影响 PDL 细胞的存活和代谢活性。定量表达分析表明,长期培养 TNF 会损害早期和晚期发育阶段的成骨细胞命运。此外,TNF明显降低了PDL细胞的基质分泌:本研究数据证实 TNF 是促炎性重塑的调节因子,它影响分化行为,但不影响牙周的新陈代谢和细胞增殖。因此,TNF 是调节牙周正畸重塑过程的一个有趣靶点。
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来源期刊
CiteScore
3.90
自引率
0.00%
发文量
64
审稿时长
>12 weeks
期刊介绍: The Journal of Orofacial Orthopedics provides orthodontists and dentists who are also actively interested in orthodontics, whether in university clinics or private practice, with highly authoritative and up-to-date information based on experimental and clinical research. The journal is one of the leading publications for the promulgation of the results of original work both in the areas of scientific and clinical orthodontics and related areas. All articles undergo peer review before publication. The German Society of Orthodontics (DGKFO) also publishes in the journal important communications, statements and announcements.
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