Association of serum biomarkers level transforming growth factor-β and tumor necrosis factor-α with diabetic neuropathy.

Q3 Medicine

Human Antibodies Pub Date : 2024-01-01 DOI:10.3233/HAB-240031

Dhifaf Abdulrhaman, Hayfaa Fahad, Nawar Khalil

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引用次数: 0

Abstract

Background: Many studies have examined the role of inflammation in the development of diabetic neuropathy (DPN).

Objective: Evaluate the relation of the serum level of Transforming Growth Factor-β and Tumor Necrosis Factor-α and development of diabetic peripheral neuropathy DPN.

Methods: In a case-control study, randomly selected 140 diabetic patients were included, the randomly selected patients were divided equally and matched into a case group who have diabetic peripheral neuropathy and diabetic neuropathy-free patients as a control group. For both groups whole blood sample was examined to compare for (TGF-β), and (TNF-α) levels determination by ELISA technique.

Results: The age of the study samples ranged from 25 to 80 years with a male ratio of 1.45:1 although the sex differences between both groups were not significant. The mean levels of (TNF-α) and (TGF-β) was significantly higher among cases group than that of controls group (254.86 ± 75.9 vs158.01 ± 50.600) for TNF-α and for TGF- β (312.85 ± 62.27 vs. 217.82 ± 52.95) respectively. Both TNF-α and TGF-β have high sensitivity and specificity in detection of DPN. The sensitivity of TNF-α was 95.7% and specificity of 61.4% area under the ROC curve (AUC) of 0.870 ± 0.029, while the sensitivity of TGF-β was 91.4%, and specificity of 67.1 with good area under the ROC curve (AUC) of 0.891 ± 0.026 (P=0.000).

Conclusions: TNF-α and TGF -β are significantly elevated levels in patients with DPN, these cytokines could be used as indicators for the development of DPN.

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血清生物标志物水平转化生长因子-β和肿瘤坏死因子-α与糖尿病神经病变的关系

背景：许多研究都探讨了炎症在糖尿病神经病变（DPN）发生过程中的作用：许多研究都探讨了炎症在糖尿病神经病变（DPN）发生中的作用：评估血清中转化生长因子-β和肿瘤坏死因子-α的水平与糖尿病周围神经病变DPN发病的关系：在一项病例对照研究中，随机抽取 140 名糖尿病患者，将其平均分为患有糖尿病周围神经病变的病例组和无糖尿病周围神经病变的对照组。对两组患者的全血样本进行检测，通过 ELISA 技术比较（TGF-β）和（TNF-α）水平：研究样本的年龄从 25 岁到 80 岁不等，男女比例为 1.45:1，但两组样本的性别差异并不明显。病例组（TNF-α）和（TGF-β）的平均水平明显高于对照组（TNF-α为 254.86 ± 75.9 vs 158.01 ± 50.600，TGF- β为 312.85 ± 62.27 vs 217.82 ± 52.95）。TNF-α和TGF-β在检测DPN方面都具有较高的灵敏度和特异性。TNF-α的灵敏度为95.7%，特异性为61.4%，ROC曲线下面积（AUC）为0.870 ± 0.029；TGF-β的灵敏度为91.4%，特异性为67.1，ROC曲线下面积（AUC）为0.891 ± 0.026（P=0.000）：结论：TNF-α和TGF-β在DPN患者中明显升高，这些细胞因子可作为DPN发病的指标。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Human Antibodies Medicine-Immunology and Allergy

CiteScore

3.50

自引率

0.00%

发文量

期刊介绍： Human Antibodies is an international journal designed to bring together all aspects of human hybridomas and antibody technology under a single, cohesive theme. This includes fundamental research, applied science and clinical applications. Emphasis in the published articles is on antisera, monoclonal antibodies, fusion partners, EBV transformation, transfections, in vitro immunization, defined antigens, tissue reactivity, scale-up production, chimeric antibodies, autoimmunity, natural antibodies/immune response, anti-idiotypes, and hybridomas secreting interesting growth factors. Immunoregulatory molecules, including T cell hybridomas, will also be featured.