Short-term impact of vitamin K2 supplementation on biochemical parameters and lipoprotein fractions.

IF 3 3区 生物学 Q2 BIOCHEMICAL RESEARCH METHODS
Milos Barna, Katerina Dunovska, Jana Cepova, Julia Werle, Richard Prusa, Geir Bjørklud, Pavel Melichercik, Rene Kizek, Eva Klapkova
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Abstract

This study explored the short-term effects of vitamin K2 (VK2) supplementation on biochemical parameters (vitamin D, vitamin E, vitamin A, alkaline phosphatase, calcium, phosphorus (P), magnesium, metallothionein, triglycerides, cholesterol, high-density lipoprotein (HDL), low-density lipoprotein (LDL), and lipoprotein fractions (albumin, HDL, very low-density lipoprotein (VLDL), LDL, and chylomicrons). A short-term experiment (24 h, six probands) was performed to track changes in VK2 levels after a single-dose intake (360 µg/day). Liquid chromatography-tandem mass spectrometry was used to monitor vitamin K levels (menaquinone-4 (MK-4), menaquinone-7 (MK-7), and vitamin K1 [VK1]) with a limit of detection of 1.9 pg/mL for VK1 and 3.8 pg/mL for the two forms of VK2. Results showed that MK-7 levels significantly increased within 2-6 h post-administration and then gradually declined. MK-4 levels were initially low, showing a slight increase, whereas VK1 levels rose initially and then decreased. Biochemical analyses indicated no significant changes in sodium, chloride, potassium, calcium, magnesium, albumin, or total protein levels. A transient increase in P was observed, peaking at 12 h before returning to baseline. Agarose gel electrophoresis of lipoprotein fractions revealed distinct chylomicron bands and variations in VLDL and HDL mobility, influenced by dietary lipids and VK2 supplementation. These findings suggest effective absorption and metabolism of MK-7 with potential implications for bone metabolism and cardiovascular health.

维生素 K2 补充剂对生化指标和脂蛋白组分的短期影响。
本研究探讨了补充维生素 K2 (VK2) 对生化指标(维生素 D、维生素 E、维生素 A、碱性磷酸酶、钙、磷 (P)、镁、金属硫蛋白、甘油三酯、胆固醇、高密度脂蛋白 (HDL)、低密度脂蛋白 (LDL)、脂蛋白组分(白蛋白、高密度脂蛋白、极低密度脂蛋白)的短期影响、金属硫蛋白、甘油三酯、胆固醇、高密度脂蛋白(HDL)、低密度脂蛋白(LDL)和脂蛋白组分(白蛋白、高密度脂蛋白、极低密度脂蛋白(VLDL)、低密度脂蛋白和乳糜微粒)。进行了一项短期实验(24 小时,6 名受试者),以跟踪单剂量摄入(360 微克/天)后 VK2 水平的变化。采用液相色谱-串联质谱法监测维生素 K 水平(脑醌-4 (MK-4)、脑醌-7 (MK-7)和维生素 K1 [VK1]),VK1 的检测限为 1.9 皮克/毫升,两种形式的 VK2 的检测限为 3.8 皮克/毫升。结果显示,MK-7 的水平在给药后 2-6 小时内显著上升,然后逐渐下降。MK-4 的水平最初较低,后来略有上升,而 VK1 的水平最初上升,然后下降。生化分析表明,钠、氯、钾、钙、镁、白蛋白或总蛋白水平没有明显变化。观察到 P 有短暂的增加,在 12 小时达到峰值,然后恢复到基线水平。脂蛋白组分的琼脂糖凝胶电泳显示了不同的乳糜微粒条带以及 VLDL 和 HDL 流动性的变化,这些变化受到膳食脂类和 VK2 补充剂的影响。这些研究结果表明,MK-7 的有效吸收和代谢对骨代谢和心血管健康具有潜在的影响。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
ELECTROPHORESIS
ELECTROPHORESIS 生物-分析化学
CiteScore
6.30
自引率
13.80%
发文量
244
审稿时长
1.9 months
期刊介绍: ELECTROPHORESIS is an international journal that publishes original manuscripts on all aspects of electrophoresis, and liquid phase separations (e.g., HPLC, micro- and nano-LC, UHPLC, micro- and nano-fluidics, liquid-phase micro-extractions, etc.). Topics include new or improved analytical and preparative methods, sample preparation, development of theory, and innovative applications of electrophoretic and liquid phase separations methods in the study of nucleic acids, proteins, carbohydrates natural products, pharmaceuticals, food analysis, environmental species and other compounds of importance to the life sciences. Papers in the areas of microfluidics and proteomics, which are not limited to electrophoresis-based methods, will also be accepted for publication. Contributions focused on hyphenated and omics techniques are also of interest. Proteomics is within the scope, if related to its fundamentals and new technical approaches. Proteomics applications are only considered in particular cases. Papers describing the application of standard electrophoretic methods will not be considered. Papers on nanoanalysis intended for publication in ELECTROPHORESIS should focus on one or more of the following topics: • Nanoscale electrokinetics and phenomena related to electric double layer and/or confinement in nano-sized geometry • Single cell and subcellular analysis • Nanosensors and ultrasensitive detection aspects (e.g., involving quantum dots, "nanoelectrodes" or nanospray MS) • Nanoscale/nanopore DNA sequencing (next generation sequencing) • Micro- and nanoscale sample preparation • Nanoparticles and cells analyses by dielectrophoresis • Separation-based analysis using nanoparticles, nanotubes and nanowires.
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