Molecular testing for equine herpesviruses 1 (EHV-1) in healthy postpartum broodmares.

IF 0.9 4区 农林科学 Q3 VETERINARY SCIENCES
Luis G Arroyo, Diego E Gomez, Alison Moore, Maria Papapetrou, Brandon N Lillie
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引用次数: 0

Abstract

Objective: Our objective was to determine whether equine herpesviruses 1 (EHV-1) viral nucleic acids could be detected immediately after foaling from nasal and vaginal swabs, whole blood, and placental tissue of healthy mares.

Animals procedure and results: Nasal and vaginal swabs, EDTA blood, and placental tissue (296 samples) were collected from 74 clinically healthy postpartum broodmares within 24 h after giving birth to live, clinically healthy foals. All samples were tested (PCR) for nucleic acids of neuropathogenic and non-neuropathogenic strains of EHV-1, and all were negative.

Conclusion and clinical relevance: As EHV-1 was not detected in the immediate postpartum period in healthy mares with uncomplicated foaling, we inferred that EHV-1-positive samples from aborting mares and/or EHV-1 detection in fetal membranes indicate EHV-1-associated abortion.

健康产后母马的马疱疹病毒 1 (EHV-1) 分子检测。
目的我们的目的是确定能否在健康母马产后立即从其鼻拭子、阴道拭子、全血和胎盘组织中检测到马疱疹病毒 1 (EHV-1) 病毒核酸:从 74 头临床健康的产后母马身上采集鼻拭子、阴道拭子、EDTA 血液和胎盘组织(296 个样本),采集时间均在产下临床健康的活马驹后 24 小时内。对所有样本进行了神经致病性和非神经致病性 EHV-1 株核酸检测(PCR),结果均为阴性:结论和临床意义:由于在产后不久未并发症的健康母马中未检测到 EHV-1,我们推断流产母马的 EHV-1 阳性样本和/或胎膜中检测到 EHV-1 表明 EHV-1 相关性流产。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
CiteScore
1.20
自引率
10.00%
发文量
177
审稿时长
12-24 weeks
期刊介绍: The Canadian Veterinary Journal (CVJ) provides a forum for the discussion of all matters relevant to the veterinary profession. The mission of the Journal is to educate by informing readers of progress in clinical veterinary medicine, clinical veterinary research, and related fields of endeavor. The key objective of The CVJ is to promote the art and science of veterinary medicine and the betterment of animal health. A report suggesting that animals have been unnecessarily subjected to adverse, stressful, or harsh conditions or treatments will not be processed for publication. Experimental studies using animals will only be considered for publication if the studies have been approved by an institutional animal care committee, or equivalent, and the guidelines of the Canadian Council on Animal Care, or equivalent, have been followed by the author(s).
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