Differential methylation patterns in paternally imprinted gene promoter regions in sperm from hepatitis B virus infected individuals.

IF 2.4 3区生物学 Q4 CELL BIOLOGY

BMC Molecular and Cell Biology Pub Date : 2024-08-01 DOI:10.1186/s12860-024-00515-7

Baoyan Wu, Yuying Sheng, Wenwei Yu, Lewen Ruan, Hao Geng, Chuan Xu, Chao Wang, Dongdong Tang, Mingrong Lv, Rong Hua, Kuokuo Li

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Abstract

Background: Hepatitis B virus (HBV) infection poses a substantial threat to human health, impacting not only infected individuals but also potentially exerting adverse effects on the health of their offspring. The underlying mechanisms driving this phenomenon remain elusive. This study aims to shed light on this issue by examining alterations in paternally imprinted genes within sperm.

Methods: A cohort of 35 individuals with normal semen analysis, comprising 17 hepatitis B surface antigen (HBsAg)-positive and 18 negative individuals, was recruited. Based on the previous research and the Online Mendelian Inheritance in Man database (OMIM, https://www.omim.org/ ), targeted promoter methylation sequencing was employed to investigate 28 paternally imprinted genes associated with various diseases.

Results: Bioinformatic analyses revealed 42 differentially methylated sites across 29 CpG islands within 19 genes and four differentially methylated CpG islands within four genes. At the gene level, an increase in methylation of DNMT1 and a decrease in methylation of CUL7, PRKAG2, and TP53 were observed. DNA methylation haplotype analysis identified 51 differentially methylated haplotypes within 36 CpG islands across 22 genes.

Conclusions: This is the first study to explore the effects of HBV infection on sperm DNA methylation and the potential underlying mechanisms of intergenerational influence of paternal HBV infection.

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乙型肝炎病毒感染者精子中父系印记基因启动子区域的差异甲基化模式。

背景：乙型肝炎病毒（HBV）感染对人类健康构成严重威胁，不仅影响感染者，还可能对其后代的健康产生不利影响。驱动这一现象的潜在机制仍然难以捉摸。本研究旨在通过检测精子中父系印记基因的改变来揭示这一问题：方法：研究人员招募了 35 名精液分析正常的个体，包括 17 名乙肝表面抗原（HBsAg）阳性个体和 18 名阴性个体。根据先前的研究和在线人类孟德尔遗传数据库（OMIM，https://www.omim.org/ ），采用靶向启动子甲基化测序研究了与各种疾病相关的28个父系印迹基因：生物信息学分析发现，19 个基因的 29 个 CpG 岛上有 42 个不同的甲基化位点，4 个基因的 4 个 CpG 岛上有不同的甲基化位点。在基因水平上，观察到 DNMT1 的甲基化增加，CUL7、PRKAG2 和 TP53 的甲基化减少。DNA 甲基化单倍型分析在 22 个基因的 36 个 CpG 岛中发现了 51 个不同的甲基化单倍型：这是第一项探讨 HBV 感染对精子 DNA 甲基化的影响以及父系 HBV 感染对代际影响的潜在机制的研究。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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