Feasibility of a Fiber-Dispersive Raman Spectrometer for Biomarker Detection.

IF 2.2 3区 化学 Q2 INSTRUMENTS & INSTRUMENTATION
Applied Spectroscopy Pub Date : 2024-10-01 Epub Date: 2024-08-18 DOI:10.1177/00037028241267892
Mariia Sidorova, Sergey G Pavlov, Ute Böttger, Mickael Baqué, Alexei D Semenov, Heinz-Wilhelm Hübers
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引用次数: 0

Abstract

Raman spectroscopy is among the top analytical techniques for ultra-low-dense organic matter, crucial to the search for life and analysis of celestial body surfaces in space exploration missions. Achieving the ultimate sensitivity of in-situ Raman spectroscopy necessitates a breakthrough in detecting inelastically scattered light. Single-photon detectors (SPDs) operating in photon counting mode, which can differentiate between Raman and luminescence responses, are promising candidates for the challenging scientific requirements. Since large SPD arrays are not yet commercially available, a dispersive element can be adapted to a single-pixel detector. By exploiting chromatic dispersion in optical fibers and picosecond-pulsed excitation, we delay the arrivals of different spectral components onto a single-pixel SPD. This method also separates weak Raman signals from stronger luminescence through correlated time-domain measurements. We study the impact of fiber properties and the excitation wavelength of a pulsed laser on the spectral resolution of the fiber-dispersive Raman spectrometer (FDRS). Additionally, we demonstrate the FDRS's potential for studying biomarkers and discuss its feasibility for analyzing inclusions in ice matrices.

EXPRESS:用于生物标记检测的光纤色散拉曼光谱仪的可行性。
拉曼光谱是超低密度有机物的顶级分析技术之一,对于太空探索任务中寻找生命和分析天体表面至关重要。要实现原位拉曼光谱的终极灵敏度,就必须在非弹性散射光的探测方面取得突破。在光子计数模式下工作的单光子探测器(SPD)可以区分拉曼和发光响应,是满足具有挑战性的科学要求的理想选择。由于大型 SPD 阵列尚未投入市场,因此可将色散元件改装成单像素探测器。通过利用光纤中的色度色散和皮秒脉冲激发,我们可以延迟不同光谱成分到达单像素 SPD 的时间。这种方法还能通过相关时域测量将微弱的拉曼信号与较强的发光信号分离开来。我们研究了光纤特性和脉冲激光器的激发波长对光纤色散拉曼光谱仪(FDRS)光谱分辨率的影响。此外,我们还展示了光纤色散拉曼光谱仪在研究生物标记物方面的潜力,并讨论了它在分析冰基质中夹杂物方面的可行性。
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来源期刊
Applied Spectroscopy
Applied Spectroscopy 工程技术-光谱学
CiteScore
6.60
自引率
5.70%
发文量
139
审稿时长
3.5 months
期刊介绍: Applied Spectroscopy is one of the world''s leading spectroscopy journals, publishing high-quality peer-reviewed articles, both fundamental and applied, covering all aspects of spectroscopy. Established in 1951, the journal is owned by the Society for Applied Spectroscopy and is published monthly. The journal is dedicated to fulfilling the mission of the Society to “…advance and disseminate knowledge and information concerning the art and science of spectroscopy and other allied sciences.”
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