Microbiological Characteristics of Clinically Isolated Staphylococcus aureus with Different Hemolytic Phenotypes in China

IF 2.9 3区医学 Q2 INFECTIOUS DISEASES

Infection and Drug Resistance Pub Date : 2024-08-01 DOI:10.2147/idr.s466416

Wei Tang, Ying Liu, Xin Li, Guiyun Leng, Ju Gao, Yawu Wang, Jie Yao, Zhou Liu, Qiang Zhou, Yuanhong Xu

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Abstract

Purpose: This study aimed to investigate the microbiological characteristics of clinically isolated Staphylococcus aureus with different hemolytic phenotypes in China.
Materials and Methods: Using the three-point inoculation method, the hemolytic phenotypes of 1295 clinically isolated S. aureus strains were detected and categorized. Antimicrobial susceptibility testing of all strains was performed using a VITEK 2 Compact System. After sample size matching, plasma coagulase activity, catalase activity, mRNA expression of hemolysin genes (hla, hlb, hlc, and hld), biofilm formation, growth kinetics, inflammatory response of macrophages and cytotoxicity of S. aureus with different hemolytic phenotypes using the rabbit plasma kit, the catalase test on slides, qRT-PCR, crystal violet staining, the microcultivation assay, the ELISA kits, and the CCK-8 assay, respectively.
Results: Seven categories of hemolytic phenotypes were identified. Accordingly, strains were categorized into seven different groups, including S. aureus with complete hemolytic phenotype (SCHP), S. aureus with weak hemolytic phenotype (SWHP), S. aureus with incomplete hemolytic phenotype 1 (SIHP-1), SIHP-2, SIHP-3, SIHP-4 and SIHP-5, the last three of which were reported for the first time. Except for the hemolytic phenotype, all seven groups differed in clinical isolation rates, antibiotic resistance profile, plasma coagulase activity, mRNA expression of hemolysin genes, biofilm formation, growth kinetics, inflammatory response of macrophages, and cytotoxicity.
Conclusion: S. aureus with different hemolytic phenotypes have distinctive microbiological characteristics. Clinical microbiologists need to be vigilant about the hemolytic phenotypes when culturing S. aureus strains, and actively enhance communication with clinicians to optimize the treatment of infection.

Keywords: Staphylococcus aureus, hemolytic phenotype, hemolysin, microbiological characteristics, antibiotic resistance

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中国临床分离的不同溶血型金黄色葡萄球菌的微生物学特征

目的：本研究旨在探讨中国临床分离的不同溶血表型金黄色葡萄球菌的微生物学特征：采用三点接种法，对 1295 株临床分离的金黄色葡萄球菌的溶血表型进行检测和分类。使用 VITEK 2 Compact 系统对所有菌株进行抗菌药敏感性检测。样本量匹配后，对不同溶血菌株的血浆凝固酶活性、过氧化氢酶活性、溶血素基因（hla、hlb、hlc 和 hld）的 mRNA 表达、生物膜形成、生长动力学、巨噬细胞的炎症反应和细胞毒性进行了检测。分别使用兔血浆试剂盒、玻片过氧化氢酶试验、qRT-PCR、结晶紫染色、微培养试验、ELISA试剂盒和CCK-8检测法检测了不同溶血表型的金黄色葡萄球菌：结果：确定了七类溶血表型。因此，菌株被分为七组，包括完全溶血表型金黄色葡萄球菌（SCHP）、弱溶血表型金黄色葡萄球菌（SWHP）、不完全溶血表型金黄色葡萄球菌1（SIHP-1）、SIHP-2、SIHP-3、SIHP-4和SIHP-5，其中后三组为首次报道。除溶血表型外，所有七组在临床分离率、抗生素耐药性、血浆凝固酶活性、溶血素基因 mRNA 表达、生物膜形成、生长动力学、巨噬细胞炎症反应和细胞毒性方面均存在差异：结论：不同溶血表型的金黄色葡萄球菌具有不同的微生物学特征。临床微生物学家在培养金黄色葡萄球菌菌株时需要对溶血表型保持警惕，并积极加强与临床医生的沟通，优化感染治疗：金黄色葡萄球菌溶血表型溶血素微生物学特征抗生素耐药性

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来源期刊

Infection and Drug Resistance Medicine-Pharmacology (medical)

CiteScore

5.60

自引率

7.70%

发文量

826

审稿时长

16 weeks

期刊介绍： About Journal Editors Peer Reviewers Articles Article Publishing Charges Aims and Scope Call For Papers ISSN: 1178-6973 Editor-in-Chief: Professor Suresh Antony An international, peer-reviewed, open access journal that focuses on the optimal treatment of infection (bacterial, fungal and viral) and the development and institution of preventative strategies to minimize the development and spread of resistance.

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