Serum SIRT3 levels in epilepsy patients and its association with clinical outcomes and severity: A prospective observational study

IF 1.7 4区医学 Q2 MEDICINE, GENERAL & INTERNAL

Open Medicine Pub Date : 2024-07-30 DOI:10.1515/med-2024-1011

Yun Hu, Ting Zhou, Qingye Li

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引用次数: 0

Abstract

Objective In this prospective observational study, we aimed to investigate the serum levels of sirtuin (SIRT)3 in epilepsy patients and its association with the severity of the disease. Methods This prospective observational study included 203 patients with symptomatic epilepsy and 100 healthy controls who visited our hospital from November 2019 to November 2022. The severity of the disease in epilepsy patients was assessed using the National Hospital Seizure Severity Scale (NHS3). We used enzyme-linked immunosorbent assay to measure the serum levels of SIRT3, interleukin (IL)-6, IL-1β, tumor necrosis factor-alpha, and C-reactive protein in all patients. In addition, the cognitive function of all study participants was evaluated using the Mini-Mental State Examination and the Montreal Cognitive Assessment (MOCA). All data were analyzed using SPSS 25.0 software. Results The MOCA scores of the epilepsy patients were significantly lower compared to the healthy volunteers (P < 0.05). The serum SIRT3 levels were decreased significantly in patients with refractory epilepsy (183.16 ± 17.22 pg/mL) compared to non-refractory epilepsy patients (199.00 ± 18.68 pg/mL). In addition, serum SIRT3 levels were negatively correlated with the inflammatory factors IL-6 (Pearson’s correlation −0.221, P = 0.002) and NHS score (Pearson’s correlation −0.272, P < 0.001) of epilepsy patients, while positively correlated with MOCA scores (Pearson’s correlation 0.166, P = 0.018). Furthermore, the receiver operating characteristic curve demonstrated that serum SIRT3 could be used to diagnose epilepsy, as well as refractory epilepsy. Finally, logistic regression analysis showed that SIRT3 (OR = 1.028, 95%CI: 1.003–1.054, P = 0.028), IL-6 (OR = 0.666, 95%CI: 0.554–0.800, P < 0.001), IL-1β (OR = 0.750, 95%CI: 0.630–0.894, P = 0.001), and NHS3 (OR = 0.555, 95%CI: 0.435–0.706, P < 0.001) were risk factors for refractory epilepsy. Conclusion In conclusion, our findings demonstrated that serum SIRT3 levels were significantly decreased in epilepsy patients and further decreased in patients with refractory epilepsy. This study might provide new therapeutic targets and comprehensive treatment strategies for epilepsy patients.

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癫痫患者的血清 SIRT3 水平及其与临床结果和严重程度的关系：前瞻性观察研究

目的在这项前瞻性观察研究中，我们旨在调查癫痫患者血清中sirtuin（SIRT）3的水平及其与疾病严重程度的关系。方法这项前瞻性观察研究纳入了2019年11月至2022年11月期间到我院就诊的203名症状性癫痫患者和100名健康对照者。采用全国医院癫痫发作严重程度量表（NHS3）评估癫痫患者的疾病严重程度。我们采用酶联免疫吸附法测定了所有患者血清中SIRT3、白细胞介素（IL）-6、IL-1β、肿瘤坏死因子-α和C反应蛋白的水平。此外，还使用迷你精神状态检查和蒙特利尔认知评估（MOCA）对所有研究参与者的认知功能进行了评估。所有数据均使用 SPSS 25.0 软件进行分析。结果癫痫患者的 MOCA 评分明显低于健康志愿者（P < 0.05）。与非难治性癫痫患者（199.00 ± 18.68 pg/mL）相比，难治性癫痫患者的血清 SIRT3 水平明显下降（183.16 ± 17.22 pg/mL）。此外，血清 SIRT3 水平与癫痫患者的炎症因子 IL-6 （Pearson's correlation -0.221，P = 0.002）和 NHS 评分（Pearson's correlation -0.272，P < 0.001）呈负相关，而与 MOCA 评分呈正相关（Pearson's correlation 0.166，P = 0.018）。此外，接收者操作特征曲线显示，血清 SIRT3 可用于诊断癫痫和难治性癫痫。最后，逻辑回归分析表明，SIRT3（OR = 1.028，95%CI：1.003-1.054，P = 0.028）、IL-6（OR = 0.666，95%CI：0.554-0.800，P < 0.001）、IL-1β（OR = 0.750，95%CI：0.630-0.894，P = 0.001）和 NHS3（OR = 0.555，95%CI：0.435-0.706，P < 0.001）是难治性癫痫的危险因素。结论总之，我们的研究结果表明，癫痫患者的血清 SIRT3 水平显著降低，难治性癫痫患者的 SIRT3 水平进一步降低。这项研究可为癫痫患者提供新的治疗靶点和综合治疗策略。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Open Medicine Medicine-General Medicine

CiteScore

3.00

自引率

0.00%

发文量

153

审稿时长

20 weeks

期刊介绍： Open Medicine is an open access journal that provides users with free, instant, and continued access to all content worldwide. The primary goal of the journal has always been a focus on maintaining the high quality of its published content. Its mission is to facilitate the exchange of ideas between medical science researchers from different countries. Papers connected to all fields of medicine and public health are welcomed. Open Medicine accepts submissions of research articles, reviews, case reports, letters to editor and book reviews.