Elicitation boosts withaferin A and withanolide A production in Withania somnifera (L.) Dunal hairy root cultures

IF 2.3 3区 生物学 Q3 BIOTECHNOLOGY & APPLIED MICROBIOLOGY
Ganesan Mahendran, Sornkanok Vimolmangkang
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Abstract

Withania somnifera (L.) Dunal, an Ayurvedic herb rich in withanosides, has anti-cancer and health-rejuvenating properties. These bioactive chemicals, primarily found in the roots, are of significant interest for production in hairy root cultures. In this study, researchers investigated a hairy root line produced by Rhizobium rhizogenes (ATCC 43,057), varying cell density, infection period, and acetosyringone concentrations. The most successful root induction frequency (31.66% ± 2.98%) occurred at a bacterial cell density of OD600 = 0.8 combined with a 20-min infection period and 200 µM acetosyringone. After 25 days, the hairy root culture achieved its highest biomass (5.20 ± 0.15 g/50 mL flask fresh weight (FW)) in MS liquid medium with 3% sucrose. At 30 and 25 days, the greatest amounts of withanolide A (0.824 ± 0.01 mg/g dry weight (DW) and withaferin A (1.292 ± 0.02 mg/g DW) were observed. Hairy roots were further treated with methyl jasmonate (MJ) and salicylic acid (SA) at various concentrations (25, 50, and 100 µM). Notably, the maximum content of withanolide A (2.90 ± 0.08 mg/g DW, 3.86-fold) and withaferin A (3.40 ± 0.01 mg/g DW, 2.61-fold) were obtained in the hairy roots treated with MJ at 100 µM. After 48 h, the content of withanolide A reached 3.16 ± 0.03 mg/g DW (6.72-fold), and withaferin A peaked at 4.32 ± 0.05 mg/g DW (4.59-fold) when treated with 50 µM SA + 100 µM MJ. This study demonstrated that W. somnifera hairy root culture enhances large-scale production of withanolide A and withaferin A.

Abstract Image

诱导可提高睡茄(L. )Dunal 毛根培养物中的睡茄素 A 和睡茄内酯 A 的产量
睡茄(Withania somnifera (L.) Dunal)是一种阿育吠陀草药,富含睡茄苷,具有抗癌和恢复健康的功效。这些生物活性化学物质主要存在于根部,对毛根培养物的生产具有重要意义。在这项研究中,研究人员调查了由根瘤根瘤菌(ATCC 43,057)产生的毛细根系,并改变了细胞密度、感染期和乙酰丁香酮浓度。当细菌细胞密度为 OD600 = 0.8、感染期为 20 分钟、乙酰丁香酮浓度为 200 µM 时,诱导生根的成功率最高(31.66% ± 2.98%)。25 天后,发根培养物在含 3% 蔗糖的 MS 液体培养基中达到最高生物量(5.20 ± 0.15 g/50 mL 烧瓶鲜重 (FW))。在 30 天和 25 天时,观察到了最大量的随花色素 A(0.824 ± 0.01 毫克/克干重)和随花色素 A(1.292 ± 0.02 毫克/克干重)。用不同浓度(25、50 和 100 µM)的茉莉酸甲酯(MJ)和水杨酸(SA)进一步处理毛根。值得注意的是,用 100 µM 的 MJ 处理的毛细根中,随花色素 A 的含量最高(2.90 ± 0.08 mg/g(干重),3.86 倍),随花色素 A 的含量最高(3.40 ± 0.01 mg/g(干重),2.61 倍)。48 小时后,用 50 µM SA + 100 µM MJ 处理的毛细根中,随花色素 A 的含量达到 3.16 ± 0.03 mg/g DW(6.72 倍),随花色素 A 的含量达到峰值 4.32 ± 0.05 mg/g DW(4.59 倍)。这项研究表明,桑尼佛拉毛根培养可提高大规模生产的睡茄素 A 和睡茄素 A。
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来源期刊
Plant Cell, Tissue and Organ Culture
Plant Cell, Tissue and Organ Culture 生物-生物工程与应用微生物
CiteScore
5.40
自引率
13.30%
发文量
203
审稿时长
3.3 months
期刊介绍: This journal highlights the myriad breakthrough technologies and discoveries in plant biology and biotechnology. Plant Cell, Tissue and Organ Culture (PCTOC: Journal of Plant Biotechnology) details high-throughput analysis of gene function and expression, gene silencing and overexpression analyses, RNAi, siRNA, and miRNA studies, and much more. It examines the transcriptional and/or translational events involved in gene regulation as well as those molecular controls involved in morphogenesis of plant cells and tissues. The journal also covers practical and applied plant biotechnology, including regeneration, organogenesis and somatic embryogenesis, gene transfer, gene flow, secondary metabolites, metabolic engineering, and impact of transgene(s) dissemination into managed and unmanaged plant systems.
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