Investigation of the anticancer of photodynamic therapy effects by using the novel Schiff base ligand palladium complexes on human breast cancer cell line

IF 2.2 4区 化学 Q2 Engineering
Burcu Demirbağ, Ebru Ballı, Metin Yıldırım, Hakan Ünver, Gülhan Temel, Mustafa Kemal Yılmaz, Evren Değirmenci, Deniz Kibar
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Abstract

Chemotherapy plays a role in many cancer therapies, including breast cancer, but due to its significant side effects, alternate treatment approaches have been investigated. One such alternative is photodynamic therapy (PDT), which employs a combination of oxygen, a photosensitizer (PS), and light of a specific wavelength. Transition metal complexes and SBL have gained interest in PDT. In this study aimed to assess the potential antitumor activity and underlying mechanism of a newly synthesized Schiff base ligand (SBL)-mediated PDT on MCF-7 cells, comparing its efficacy to cisplatin. We synthesized and characterized two novel Pd-conjugated SBL compounds (complex-1 and 2). Following the treatment of MCF-7 cells with these compounds, a light-emitting diode (LED) was utilized to deliver a light dose of 14.8 J/cm2. In MCF-7 cells, we also looked at cytotoxicity, cell death rates, and ROS levels. Results indicated a significant increase in cytotoxicity (IC50:12.5 µM) (p < 0.05) and ROS levels (258.33%) (p < 0.05) in the group treated with complex-1-mediated PDT compared to control. The late apoptotic (29.78%) (p < 0.05) and necrotic (24.23%) (p < 0.05) cell death also increased significantly compared to the control group (1.86 ± 0.92, 1.89 ± 0.32, respectively). In conclusion, our study suggests that the complex-1 compound may serve as a promising candidate for anticancer agents in PDT for MCF-7 cells. In contrast, complex-2-mediated PDT did not demonstrate any observable anticancer activity.

Abstract Image

Abstract Image

利用新型希夫碱配体钯配合物对人类乳腺癌细胞系进行光动力疗法的抗癌效果研究
化疗在包括乳腺癌在内的许多癌症疗法中都扮演着重要角色,但由于其副作用较大,人们开始研究其他治疗方法。其中一种替代方法是光动力疗法(PDT),它将氧气、光敏剂(PS)和特定波长的光结合在一起使用。过渡金属复合物和 SBL 在光动力疗法中备受关注。本研究旨在评估一种新合成的希夫碱配体(SBL)介导的 PDT 对 MCF-7 细胞的潜在抗肿瘤活性及其基本机制,并将其疗效与顺铂进行比较。我们合成并鉴定了两种新型钯结合 SBL 化合物(复合物-1 和 2)。用这些化合物处理 MCF-7 细胞后,利用发光二极管 (LED) 发出 14.8 J/cm2 的光剂量。我们还检测了 MCF-7 细胞的细胞毒性、细胞死亡率和 ROS 水平。结果表明,与对照组相比,使用 complex-1 介导的光导治疗组的细胞毒性(IC50:12.5 µM)(p < 0.05)和 ROS 水平(258.33%)(p < 0.05)明显增加。与对照组相比,晚期细胞凋亡(29.78%)(p <0.05)和坏死(24.23%)(p <0.05)也显著增加(分别为 1.86 ± 0.92、1.89 ± 0.32)。总之,我们的研究表明,复合物-1化合物可能是MCF-7细胞PDT中一种有前途的抗癌候选药物。相比之下,复合物-2 介导的光导照相疗法没有显示出任何可观察到的抗癌活性。
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来源期刊
Chemical Papers
Chemical Papers Chemical Engineering-General Chemical Engineering
CiteScore
3.30
自引率
4.50%
发文量
590
期刊介绍: Chemical Papers is a peer-reviewed, international journal devoted to basic and applied chemical research. It has a broad scope covering the chemical sciences, but favors interdisciplinary research and studies that bring chemistry together with other disciplines.
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