{"title":"Harnessing the Manipulation of Single Cells to Construct Biological Structures: Tools and Applications","authors":"Congying Liu, Yuhe Chen, Rui Tong, Ziyang Wang, Deyuan Zhang, Huawei Chen, Pengfei Zhang","doi":"10.1002/adfm.202316357","DOIUrl":null,"url":null,"abstract":"<p>Artificial biological structures hold the promise for modeling cellular assembly in vitro and have advanced considerable studies in cell biology, disease modeling, drug testing, and regenerative medicine. Biological functions are derived from micro- and macroscale interactions of various cell types, and a structural property matching the tissue in vivo is required to enable precision biological function. Despite various types of tissues and organs are successfully constructed by conventional biofabrication technologies, they mostly only show a small fraction of structural features found in real tissues. Tools for single-cell manipulation provide the approach to fabricate artificial tissues cell-by-cell, and have enabled the construction of biological structures with single-cell and heterogeneous features, recapitulating the complexity in vivo. This review presents a comprehensive overview of the construction of biological structures through manipulating single cells, covering single-cell technologies with operation principles and main advances, biological structures associated with informative explanations of single-cell manipulation during construction, and representative applications mainly focusing on analysis and modeling. Current challenges and future perspectives in this field are also discussed.</p>","PeriodicalId":112,"journal":{"name":"Advanced Functional Materials","volume":null,"pages":null},"PeriodicalIF":18.5000,"publicationDate":"2024-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Advanced Functional Materials","FirstCategoryId":"88","ListUrlMain":"https://onlinelibrary.wiley.com/doi/10.1002/adfm.202316357","RegionNum":1,"RegionCategory":"材料科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"CHEMISTRY, MULTIDISCIPLINARY","Score":null,"Total":0}
引用次数: 0
Abstract
Artificial biological structures hold the promise for modeling cellular assembly in vitro and have advanced considerable studies in cell biology, disease modeling, drug testing, and regenerative medicine. Biological functions are derived from micro- and macroscale interactions of various cell types, and a structural property matching the tissue in vivo is required to enable precision biological function. Despite various types of tissues and organs are successfully constructed by conventional biofabrication technologies, they mostly only show a small fraction of structural features found in real tissues. Tools for single-cell manipulation provide the approach to fabricate artificial tissues cell-by-cell, and have enabled the construction of biological structures with single-cell and heterogeneous features, recapitulating the complexity in vivo. This review presents a comprehensive overview of the construction of biological structures through manipulating single cells, covering single-cell technologies with operation principles and main advances, biological structures associated with informative explanations of single-cell manipulation during construction, and representative applications mainly focusing on analysis and modeling. Current challenges and future perspectives in this field are also discussed.
期刊介绍:
Firmly established as a top-tier materials science journal, Advanced Functional Materials reports breakthrough research in all aspects of materials science, including nanotechnology, chemistry, physics, and biology every week.
Advanced Functional Materials is known for its rapid and fair peer review, quality content, and high impact, making it the first choice of the international materials science community.