Pro-Oxidant/ Antioxidant Balance Correlates with Red Blood Cell Indices and Anemia Severity in the Anemic Patients.

IF 1.6 Q4 BIOCHEMISTRY & MOLECULAR BIOLOGY
Soheil Mohammad Shafiee, Fatemeh Amiri, Faezeh Yousefi, Negar Akbari Nasab
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Abstract

Background: The oxidant/ antioxidant balance is disrupted in anemia. Antioxidant capacity depends on antioxidant enzyme activity and some trace elements. This study aimed to evaluate oxidant/ antioxidant status and its correlation with red blood cell indices and anemia severity in anemic patients.

Methods: Blood samples were taken from 90 anemic patients and 95 healthy people. Circulatory miR-122 was assayed by real-time PCR. Malondialdehyde (MDA), pro-oxidant/ antioxidant balance (PAB), supper oxide demitasse (SOD), glutathione peroxidase (GPxs) activity, total antioxidant capacity (TAC), and zinc were measured by colorimetric method. Selenium was also determined using atomic absorption.

Results: Selenium and zinc decreased significantly in the case group (**P=0.004 and ***P=0.000). The amount of miR-122 up-regulated in the anemia (**P=0.003). MDA was significantly raised in the case vs control (***P=0.0002). PAB was higher in the case group (**P=0.005). SOD and GPxs activity was decreased along with TAC in anemic patients (*P=0.02, **P=0.008, *P=0.038). Zinc and PAB levels correlated with some red blood cell indices. PAB was associated with anemia severity.

Conclusions: Increased PAB and decreased zinc/selenium increased oxidant levels in anemic patients. RBC indices and anemia severity were correlated with oxidant/ antioxidant somewhere.

促氧化剂/抗氧化剂平衡与贫血患者的红细胞指数和贫血严重程度有关。
背景:贫血症会破坏氧化剂/抗氧化剂的平衡。抗氧化能力取决于抗氧化酶活性和一些微量元素。本研究旨在评估贫血患者的氧化剂/抗氧化剂状态及其与红细胞指数和贫血严重程度的相关性:方法:采集了 90 名贫血患者和 95 名健康人的血液样本。方法:抽取 90 名贫血患者和 95 名健康人的血液样本,通过实时 PCR 检测循环 miR-122。用比色法测量丙二醛(MDA)、原氧化剂/抗氧化剂平衡(PAB)、氧化还原酶(SOD)、谷胱甘肽过氧化物酶(GPxs)活性、总抗氧化能力(TAC)和锌。硒也用原子吸收法测定:结果:硒和锌在病例组明显下降(**P=0.004 和 ***P=0.000)。贫血患者的 miR-122 上调(**P=0.003)。病例组与对照组相比,MDA明显升高(***P=0.0002)。病例组的 PAB 更高(**P=0.005)。贫血患者的 SOD 和 GPx 活性随着 TAC 的降低而降低(*P=0.02,**P=0.008,*P=0.038)。锌和 PAB 水平与一些红细胞指数相关。PAB与贫血严重程度相关:结论:PAB的增加和锌/硒的减少增加了贫血患者体内的氧化剂水平。红细胞指数和贫血严重程度与某处的氧化剂/抗氧化剂有关。
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来源期刊
Reports of Biochemistry and Molecular Biology
Reports of Biochemistry and Molecular Biology BIOCHEMISTRY & MOLECULAR BIOLOGY-
CiteScore
2.80
自引率
23.50%
发文量
60
审稿时长
10 weeks
期刊介绍: The Reports of Biochemistry & Molecular Biology (RBMB) is the official journal of the Varastegan Institute for Medical Sciences and is dedicated to furthering international exchange of medical and biomedical science experience and opinion and a platform for worldwide dissemination. The RBMB is a medical journal that gives special emphasis to biochemical research and molecular biology studies. The Journal invites original and review articles, short communications, reports on experiments and clinical cases, and case reports containing new insights into any aspect of biochemistry and molecular biology that are not published or being considered for publication elsewhere. Publications are accepted in the form of reports of original research, brief communications, case reports, structured reviews, editorials, commentaries, views and perspectives, letters to authors, book reviews, resources, news, and event agenda.
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