Correlation of MicroRNA-125b, Sirtuin, and Signal Transducer and Activator of Transcription 3 with Biochemical Parameters and Risk Factors in Atherosclerosis Patients.

IF 1.6 Q4 BIOCHEMISTRY & MOLECULAR BIOLOGY
Atefeh Mirderikvand, Gholamreza Shahsavari, Alireza Moayyed Kazemi, Fatemeh Ahmadpour, Banafsheh Yalameha
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引用次数: 0

Abstract

Background: Atherosclerosis (AS) is an inflammatory disease linked to vascular events, with dysregulation of microRNA (miR)-125b, contributing to cardiovascular disease pathogenesis. Moreover, there is evidence of the involvement of signal transducer and activator of transcription 3 (STAT3) and sirtuin 6 (SIRT6) in AS. This study aimed to survey the expression levels of miR-125b, STAT3, and SIRT6 in the peripheral blood mononuclear cells (PBMCs) of AS patients and controls, and to find their correlations with biochemical parameters and risk factors.

Methods: This study included blood samples from 45 controls and 45 AS patients, with PBMCs isolated using Ficoll solution. Expression levels of miR-125b, STAT3, and SIRT6 were determined via quantitative Real Time-PCR.

Results: The findings revealed a significant increase in miR-125b levels in patients compared to controls (P = 0.017). However, alterations in STAT3 and SIRT6 expression were not significant (P> 0.05). There was no substantial relationship between miR-125b and STAT3 (P = 0.522) or SIRT6 (P = 0.88). miR-125b showed a significant relationship with atherogenic indexes and creatinine (P<0.05), while the association of SIRT6 with HDL and creatinine was significant (P<0.05). STAT3 exhibited high diagnostic power for identifying individuals at risk of heart disease and hypertension (P<0.05).

Conclusion: STAT3 can serve as a valuable biomarker for detecting AS and AS-related risk factors. miR-125b and SIRT6 may be associated with AS lipid metabolism. However, further studies with larger sample sizes are recommended to mechanistically elucidate the association of these genes.

MicroRNA-125b、Sirtuin 和信号转导和转录激活因子 3 与动脉粥样硬化患者的生化参数和风险因素的相关性。
背景:动脉粥样硬化(AS)是一种与血管事件有关的炎症性疾病,微RNA(miR)-125b的失调是心血管疾病发病机制的一个因素。此外,有证据表明信号转导及激活转录3(STAT3)和sirtuin 6(SIRT6)参与了AS的发病。本研究旨在调查强直性脊柱炎患者和对照组外周血单核细胞(PBMCs)中 miR-125b、STAT3 和 SIRT6 的表达水平,并发现它们与生化指标和危险因素的相关性:这项研究包括 45 名对照组和 45 名 AS 患者的血液样本,并使用 Ficoll 溶液分离 PBMCs。通过 Real Time-PCR 定量检测 miR-125b、STAT3 和 SIRT6 的表达水平:结果:研究结果显示,与对照组相比,患者体内的 miR-125b 水平明显升高(P = 0.017)。然而,STAT3 和 SIRT6 表达的变化并不明显(P> 0.05)。miR-125b与STAT3(P = 0.522)或SIRT6(P = 0.88)之间没有实质性关系:miR-125b和SIRT6可能与强直性脊柱炎的脂质代谢有关。不过,建议进一步开展样本量更大的研究,从机理上阐明这些基因之间的关联。
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来源期刊
Reports of Biochemistry and Molecular Biology
Reports of Biochemistry and Molecular Biology BIOCHEMISTRY & MOLECULAR BIOLOGY-
CiteScore
2.80
自引率
23.50%
发文量
60
审稿时长
10 weeks
期刊介绍: The Reports of Biochemistry & Molecular Biology (RBMB) is the official journal of the Varastegan Institute for Medical Sciences and is dedicated to furthering international exchange of medical and biomedical science experience and opinion and a platform for worldwide dissemination. The RBMB is a medical journal that gives special emphasis to biochemical research and molecular biology studies. The Journal invites original and review articles, short communications, reports on experiments and clinical cases, and case reports containing new insights into any aspect of biochemistry and molecular biology that are not published or being considered for publication elsewhere. Publications are accepted in the form of reports of original research, brief communications, case reports, structured reviews, editorials, commentaries, views and perspectives, letters to authors, book reviews, resources, news, and event agenda.
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