The Potential Association Between microRNA 135-5P and p62 and Their Effect on NRF2 Pathway in Multiple Sclerosis.

IF 1.6 Q4 BIOCHEMISTRY & MOLECULAR BIOLOGY

Reports of Biochemistry and Molecular Biology Pub Date : 2024-01-01 DOI:10.61186/rbmb.12.4.512

Azza Abusree Ahmed, Salwa Fayez Hasan, Laila Ahmed Rashed, Noura Ragab, Rania Shehata Ismail, Doaa Mostafa Gharib

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引用次数: 0

Abstract

Background: Multiple Sclerosis (MS) is a prevalent non-traumatic disabling disease affecting young adults, characterized by complexity in its pathogenesis. Nuclear factor erythroid 2-Related Factor 2 (NRF2) serves as a crucial transcriptional regulator of anti-inflammatory and antioxidant enzymes, influenced by the ubiquitous protein p62. It acts as a scaffold directing substrates to autophagosomes. This study aims to explore the potential association between microRNA 135-5p and p62 and their impact on inflammation and oxidative stress through the NRF2 pathway in MS.

Methods: The study included 30 healthy controls and 60 MS patients (relapsing-remitting and secondary progressive). Real-time PCR was employed for the detection of Nrf2, p62, miRNA135-5P, and NF-κB in serum, while p53 levels were determined using ELISA.

Results: Nrf2 and p62 expression was significantly downregulated in the MS group compared to controls. Conversely, miRNA135-5P, NF-κB expression, and P53 levels were significantly elevated in the MS group.

Conclusions: This study reveals a potential association between miRNA 135-5p and p62, indicating their role in the pathogenesis of MS. Results suggest that miRNA 135-5p and p62 may influence inflammation and oxidative stress in MS through the NRF2 pathway, potentially mediated by NF-κB and p53.

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多发性硬化症中 microRNA 135-5P 和 p62 之间的潜在关联及其对 NRF2 通路的影响

背景：多发性硬化症（MS）是一种流行于青壮年的非创伤性致残疾病，其发病机制十分复杂。核因子红细胞 2 相关因子 2（NRF2）是抗炎和抗氧化酶的重要转录调节因子，受到无处不在的蛋白质 p62 的影响。它是引导底物进入自噬体的支架。本研究旨在探讨 microRNA 135-5p 和 p62 之间的潜在关联及其通过 NRF2 通路对 MS 中炎症和氧化应激的影响：研究对象包括30名健康对照者和60名多发性硬化症患者（复发缓解型和继发性进展型）。采用实时 PCR 法检测血清中的 Nrf2、p62、miRNA135-5P 和 NF-κB，同时用酶联免疫吸附法测定 p53 水平：结果：与对照组相比，多发性硬化症组的Nrf2和p62表达明显下调。结果：与对照组相比，多发性硬化症组的 Nrf2 和 p62 表达明显下调，相反，多发性硬化症组的 miRNA135-5P、NF-κB 表达和 P53 水平明显升高：这项研究揭示了 miRNA 135-5p 与 p62 之间的潜在关联，表明它们在多发性硬化症发病机制中的作用。研究结果表明，miRNA 135-5p和p62可能通过NRF2通路影响多发性硬化症的炎症和氧化应激，NF-κB和P53可能是其介导因素。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Reports of Biochemistry and Molecular Biology BIOCHEMISTRY & MOLECULAR BIOLOGY-

CiteScore

2.80

自引率

23.50%

发文量

审稿时长

10 weeks

期刊介绍： The Reports of Biochemistry & Molecular Biology (RBMB) is the official journal of the Varastegan Institute for Medical Sciences and is dedicated to furthering international exchange of medical and biomedical science experience and opinion and a platform for worldwide dissemination. The RBMB is a medical journal that gives special emphasis to biochemical research and molecular biology studies. The Journal invites original and review articles, short communications, reports on experiments and clinical cases, and case reports containing new insights into any aspect of biochemistry and molecular biology that are not published or being considered for publication elsewhere. Publications are accepted in the form of reports of original research, brief communications, case reports, structured reviews, editorials, commentaries, views and perspectives, letters to authors, book reviews, resources, news, and event agenda.