Serum miR-23 and miR-150 Profiles as Biomarkers for Predicting Recurrence following Surgical Intervention in Colorectal Cancer Patients.

IF 1.6 Q4 BIOCHEMISTRY & MOLECULAR BIOLOGY

Reports of Biochemistry and Molecular Biology Pub Date : 2024-01-01 DOI:10.61186/rbmb.12.4.540

Saeid Mahmoudivar, Habib Zarredar, Milad Asadi, Venus Zafari, Shahriyar Hashemzadeh, Rojin Farzaneh, Touraj Asvadi Kermani

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引用次数: 0

Abstract

Background: MicroRNAs (miRNAs) play pivotal roles in post-transcriptional regulation of gene expression and have emerged as crucial regulators in cancer development, progression, and metastasis. This study aimed to assess the expression profiles of miR-23, miR-223, miR-1246, and miR-150 in serum samples obtained from colorectal cancer (CRC) patients before and three months after surgery, in comparison to a healthy control group, to explore their biomarker potential.

Methods: A total of 50 blood samples were collected from patients with CRC (pre- and post-surgery), along with 50 samples from healthy controls. The relative expression levels of miR-23, miR-223, miR-1246, and miR-150 in the serum were quantified using quantitative real-time PCR.

Results: Our findings revealed upregulated expression levels of miR-23, miR-1246, and miR-223, while miR-150 exhibited significant downregulation in the serum of CRC subjects compared to healthy controls. Receiver operating characteristic (ROC) analysis indicated that miR-23 and miR-150 could distinguish CRC cases from controls with relatively high accuracy. Moreover, three months post-surgery, miR-23, miR-1246, and miR-223 serum levels were downregulated, and miR-150 was significantly upregulated. However, no significant correlations were observed between serum levels of the studied genes and the clinical features of our patients.

Conclusions: The serum levels of miR-23 and miR-150 hold promise as potential biomarkers for the diagnosis and prognosis of CRC.

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血清 miR-23 和 miR-150 图谱作为预测结直肠癌患者手术干预后复发的生物标记物

背景：微RNA（miRNA）在基因表达的转录后调控中起着关键作用，已成为癌症发生、发展和转移的重要调控因子。本研究旨在评估结直肠癌（CRC）患者术前和术后三个月的血清样本中 miR-23、miR-223、miR-1246 和 miR-150 的表达谱，并与健康对照组进行对比，以探索其生物标志物的潜力：共收集了 50 份 CRC 患者（手术前和手术后）的血液样本，以及 50 份健康对照组样本。结果：我们的研究结果表明，miR-23、miR-223、miR-1246 和 miR-150 在血清中的相对表达水平呈上升趋势：结果：与健康对照组相比，我们的研究结果显示，miR-23、miR-1246和miR-223在CRC受试者血清中的表达水平上调，而miR-150则明显下调。接收者操作特征（ROC）分析表明，miR-23 和 miR-150 能以相对较高的准确度区分 CRC 病例和对照组。此外，手术后三个月，miR-23、miR-1246 和 miR-223 血清水平下调，而 miR-150 则显著上调。然而，所研究基因的血清水平与患者的临床特征之间没有发现明显的相关性：结论：miR-23 和 miR-150 的血清水平有望成为诊断和预后 CRC 的潜在生物标志物。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Reports of Biochemistry and Molecular Biology BIOCHEMISTRY & MOLECULAR BIOLOGY-

CiteScore

2.80

自引率

23.50%

发文量

审稿时长

10 weeks

期刊介绍： The Reports of Biochemistry & Molecular Biology (RBMB) is the official journal of the Varastegan Institute for Medical Sciences and is dedicated to furthering international exchange of medical and biomedical science experience and opinion and a platform for worldwide dissemination. The RBMB is a medical journal that gives special emphasis to biochemical research and molecular biology studies. The Journal invites original and review articles, short communications, reports on experiments and clinical cases, and case reports containing new insights into any aspect of biochemistry and molecular biology that are not published or being considered for publication elsewhere. Publications are accepted in the form of reports of original research, brief communications, case reports, structured reviews, editorials, commentaries, views and perspectives, letters to authors, book reviews, resources, news, and event agenda.