Cord blood methylation at TNFRSF17 is associated with early allergic phenotypes.

IF 3.3 4区 医学 Q3 IMMUNOLOGY
Immunologic Research Pub Date : 2024-12-01 Epub Date: 2024-07-31 DOI:10.1007/s12026-024-09524-2
Hanna Danielewicz, Artur Gurgul, Anna Dębińska, Anna Drabik-Chamerska, Lidia Hirnle, Andrzej Boznański
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引用次数: 0

Abstract

Food allergy and eczema are the earliest allergic phenotypes in childhood. These diseases could be related to either IgE-mediated or non-IgE-mediated reactions to the allergen. TNFRSF17 is a key molecule in B cell maturation and is important in both types of responses.We conducted a study comparing the relative expression and the methylation status at the TNFRSF17 in regard to the child's early atopic sensitisation and allergic phenotypes.In the recruited population of 200 women and 174 children with available clinical data (physical examination by allergist and antigen-specific IgE measurements), 78 cord blood samples were included in the gene expression analysis (relative gene expression with GAPDH as reference by RT-PCR) and 96 samples with microarray DNA methylation data (whole genome methylation profile Infinium MethylationEPIC).The altered TNFRSF17 methylation pattern in the cord blood at both single cg04453550 and mean methylation at upstream of TNFRSF17 was observed in children who developed food allergy and/or eczema in early childhood. The change in methylation profile was mirrored by the relative expression. The profile of IgE sensitisation to food and/or inhalant allergens was not significantly associated with either methylation or expression of TNFRSF17.In conclusion, methylation at the upstream sites at TNFRSF17 in the cord blood at birth is associated with food allergy and eczema early in childhood.

Abstract Image

脐带血中 TNFRSF17 的甲基化与早期过敏表型有关。
食物过敏和湿疹是儿童时期最早出现的过敏症状。这些疾病可能与 IgE 介导或非 IgE 介导的过敏原反应有关。我们进行了一项研究,比较了 TNFRSF17 的相对表达和甲基化状态与儿童早期特应性过敏和过敏表型的关系。在所招募的 200 名妇女和 174 名儿童中,有可用的临床数据(过敏学家的体格检查和抗原特异性 IgE 测量),78 份脐带血样本被纳入基因表达分析(通过 RT-PCR 以 GAPDH 为参照的相对基因表达),96 份样本被纳入微阵列 DNA 甲基化数据(全基因组甲基化图谱 Infinium MethylationEPIC)。在幼儿期患食物过敏和/或湿疹的儿童中,观察到脐带血中 TNFRSF17 单个 cg04453550 甲基化模式和 TNFRSF17 上游平均甲基化模式的改变。甲基化特征的变化与相对表达量相一致。总之,出生时脐带血中 TNFRSF17 上游位点的甲基化与儿童早期的食物过敏和湿疹有关。
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来源期刊
Immunologic Research
Immunologic Research 医学-免疫学
CiteScore
6.90
自引率
0.00%
发文量
83
审稿时长
6-12 weeks
期刊介绍: IMMUNOLOGIC RESEARCH represents a unique medium for the presentation, interpretation, and clarification of complex scientific data. Information is presented in the form of interpretive synthesis reviews, original research articles, symposia, editorials, and theoretical essays. The scope of coverage extends to cellular immunology, immunogenetics, molecular and structural immunology, immunoregulation and autoimmunity, immunopathology, tumor immunology, host defense and microbial immunity, including viral immunology, immunohematology, mucosal immunity, complement, transplantation immunology, clinical immunology, neuroimmunology, immunoendocrinology, immunotoxicology, translational immunology, and history of immunology.
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