Neg1 overexpression in Trichoderma harzianum T4 enhanced its ability to produce spores and antagonistic activity against phytopathogenic fungi

IF 2.3 3区生物学 Q3 MICROBIOLOGY

Archives of Microbiology Pub Date : 2024-07-31 DOI:10.1007/s00203-024-04091-4

Jingwen Liu, Yaping Wang, Shouquan Lu, Xiaoxu Liu, Henan Zhang, Wei Wang

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Abstract

Trichoderma harzianum T4 is a soil fungus that plays an important role in the biological control of plant diseases. The aim of this study was to functionally characterize the β-1,6-glucanase gene Neg1 in T. harzianum T4 and to investigate the effect of its overexpression on biocontrol traits, especially antagonism against pathogenic fungi. We found that overexpression of Neg1 did not affect growth of T. harzianum but enhanced sporulation of T. harzianum T4 cultures. Generally, spores are closely related to the defense ability of defense fungi and can assist their proliferation and improve their colonization ability. Secondly, overexpression of Neg1 also increased the secretion level of various hydrolytic enzymes and enhanced the antagonistic ability against phytopathogenic fungi of Fusarium spp. The results suggest that Neg1 is a key gene for improving the biocontrol effect of T. harzianum T4, which contributes to a better understanding of the mechanism of action of T. harzianum T4 as a fungal biocontrol agent.

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哈茨真菌 T4 中 Neg1 的过表达增强了其产生孢子的能力和对植物病原真菌的拮抗活性。

毛霉 T4 是一种土壤真菌，在植物病害的生物防治中发挥着重要作用。本研究的目的是从功能上描述 T. harzianum T4 中的β-1,6-葡聚糖酶基因 Neg1，并研究其过度表达对生物防治性状的影响，尤其是对病原真菌的拮抗作用。我们发现，过量表达 Neg1 不会影响 T. harzianum 的生长，但会增强 T. harzianum T4 培养物的孢子生成。一般来说，孢子与防御真菌的防御能力密切相关，可以帮助真菌增殖并提高其定植能力。其次，过表达 Neg1 还能提高各种水解酶的分泌水平，增强对镰刀菌属植物病原真菌的拮抗能力。结果表明，Neg1 是提高 T. harzianum T4 生物防治效果的关键基因，有助于更好地了解 T. harzianum T4 作为真菌生物防治剂的作用机制。

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来源期刊

Archives of Microbiology 生物-微生物学

CiteScore

4.90

自引率

3.60%

发文量

601

审稿时长

3 months

期刊介绍： Research papers must make a significant and original contribution to microbiology and be of interest to a broad readership. The results of any experimental approach that meets these objectives are welcome, particularly biochemical, molecular genetic, physiological, and/or physical investigations into microbial cells and their interactions with their environments, including their eukaryotic hosts. Mini-reviews in areas of special topical interest and papers on medical microbiology, ecology and systematics, including description of novel taxa, are also published. Theoretical papers and those that report on the analysis or ''mining'' of data are acceptable in principle if new information, interpretations, or hypotheses emerge.