Effect of salt stress on plant regeneration efficiency in primed and non-primed seed's calli of rice (Oryza sativa L.) variety Swarna.

IF 1.8 4区 生物学 Q3 BIOLOGY
Sananda Mondal, Bandana Bose
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Abstract

This study leads with the primed seeds of rice (var. Swarna) with distilled water (D.W.) and various concentrations of Mg(NO3)2 (0-8 mM)/Kinetin (0-5 ppm) alone or in combination with screen out the regeneration medium induced tolerance level of NaCl. To fulfill the objective, the primed and non-primed rice seeds were inoculated in MS medium supplemented with 30 gL-1 maltose + 1 gL-1 casein hydrolysate and 2 mgL-1 of 2,4-D for callus induction and cultured up to 45 days in two sets: one set for regeneration purpose in NaCl-induced regeneration medium and another set was used to study the physiological potentiality of the callus. The 45-day-old calli were transferred into regeneration medium MSR (MS medium for regeneration) (BAP: NAA: Kinetin = 4:1:1) containing NaCl with a concentration range of 0 to 300 mM. The number of regenerating calli and shoot regeneration percentage, number of plantlets obtained from one callus, recovery of plantlets from each concentration of NaCl and proline estimation from the leaf of the regenerated plantlets were determined from one set obtained after 45 days. The calli obtained from another set after 45 days, the frequencies of total and embryogenic calli induction percentage, fresh and dry weights, proline content, nitrate reductase and superoxide dismutase activities were measured. The calli obtained from 2.5 ppm kinetin + 4 mM Mg(NO3)2 primed seeds were showed best result as compared to the other treatments for the above-mentioned parameters in different concentrations of NaCl-induced medium and survive up to 200 mM concentrations of NaCl.

盐胁迫对水稻(Oryza sativa L.)品种 Swarna 有引种和无引种胼胝体植物再生效率的影响。
本研究用蒸馏水(D.W.)和不同浓度的 Mg(NO3)2 (0-8 mM)/Kinetin(0-5 ppm)单独或混合诱导水稻(变种 Swarna)种子,以筛选出再生培养基诱导的对 NaCl 的耐受水平。为实现这一目标,将引种和未引种的水稻种子接种到添加了 30 gL-1 麦芽糖 + 1 gL-1 酪蛋白水解物和 2 mgL-1 2,4-D 的 MS 培养基中诱导胼胝体,并分两组培养至 45 天:一组用于在 NaCl 诱导的再生培养基中再生,另一组用于研究胼胝体的生理潜能。将培养 45 天的胼胝体移入再生培养基 MSR(用于再生的 MS 培养基)(BAP: NAA: Kinetin = 4:1:1),其中含有浓度范围为 0 至 300 mM 的氯化钠。从 45 天后获得的一组胼胝体中测定再生胼胝体的数量和嫩枝再生率、从一个胼胝体中获得的小植株数量、各浓度 NaCl 对小植株的回收率以及再生小植株叶片的脯氨酸含量。对 45 天后从另一组胼胝体中获得的胼胝体进行了总胼胝体诱导率和胚性胼胝体诱导率、鲜重和干重、脯氨酸含量、硝酸还原酶和超氧化物歧化酶活性的测定。与其他处理相比,在不同浓度的氯化钠诱导培养基中,2.5 ppm 的激肽+4 mM 的 Mg(NO3)2 诱导的种子所获得的胼胝体在上述参数方面表现最佳,在 200 mM 浓度的氯化钠诱导培养基中也能存活。
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来源期刊
Biologia futura
Biologia futura Agricultural and Biological Sciences-Agricultural and Biological Sciences (all)
CiteScore
3.50
自引率
0.00%
发文量
27
期刊介绍: How can the scientific knowledge we possess now influence that future? That is, the FUTURE of Earth and life − of humankind. Can we make choices in the present to change our future? How can 21st century biological research ask proper scientific questions and find solid answers? Addressing these questions is the main goal of Biologia Futura (formerly Acta Biologica Hungarica). In keeping with the name, the new mission is to focus on areas of biology where major advances are to be expected, areas of biology with strong inter-disciplinary connection and to provide new avenues for future research in biology. Biologia Futura aims to publish articles from all fields of biology.
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