Comparison of bacterial culture results obtained from three different sampling locations in dogs and cats with chronic nasal disease.

IF 1.1 4区 农林科学 Q3 VETERINARY SCIENCES
New Zealand veterinary journal Pub Date : 2024-11-01 Epub Date: 2024-07-30 DOI:10.1080/00480169.2024.2378696
T A Niedenführ, A Weickelt, G Wolf, Y Zablotski, B S Schulz
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引用次数: 0

Abstract

Aims: To assess agreement of bacterial culture results from samples taken from nasal discharge, the nasal cavity and nasal biopsy from dogs and cats with nasal disease.

Methods: Nineteen dogs and 21 cats with different nasal diseases (chronic rhinitis, n = 30; neoplasia, n = 7; sinonasal aspergillosis, n = 3) were prospectively enrolled in the study. Nasal swabs were taken bilaterally from nasal discharge at the nares, the nasal cavity, and one nasal mucosal biopsy per side. All samples were subjected to aerobic bacterial culture. Kappa statistics were used to evaluate agreement for the most prevalent bacterial species between sampling sites.

Results: A positive culture result for at least one bacterial species was detected in 80% of samples from nasal discharge/nares, 92% of nasal cavity samples, and 75% of biopsy samples. The mean agreement between the three sampling sites for positive vs. negative culture results was never greater than moderate and the precision of the estimates of agreement varied widely.The most frequently isolated bacterial species in dogs were Staphylococcus pseudintermedius, Staphylococcus spp. and Streptococcus spp. In cats, Pasteurella spp. and Staphylococcus felis were the bacterial species cultured most frequently.For the most prevalent cultured species, Staphylococcus spp., mean agreement between sites was never greater than fair and the precision again varied widely.

Conclusion: This study indicates that bacterial culture results in feline and canine nasal disease are site-specific and there was no evidence from this study for consistency between sites within a patient for many bacterial species. Consequently, if bacterial culture results from nasal swabs are used to guide therapeutic antimicrobial choice, different treatments may be selected depending on the site of culture. As a consequence, there is no evidence from this study that nasal bacterial cultures should be recommended as a routine diagnostic measure.

比较从患有慢性鼻病的狗和猫的三个不同取样位置获得的细菌培养结果。
目的:评估从患有鼻部疾病的狗和猫的鼻涕、鼻腔和鼻活检样本中提取的细菌培养结果的一致性:19只狗和21只猫患有不同的鼻部疾病(慢性鼻炎,30只;肿瘤,7只;鼻窦曲霉菌病,3只)。研究人员从双侧鼻孔、鼻腔的鼻分泌物中采集鼻拭子,每侧采集一个鼻粘膜活检样本。所有样本均进行需氧细菌培养。采用 Kappa 统计法评估各采样点之间最常见细菌种类的一致性:结果:在 80% 的鼻涕/鼻孔样本、92% 的鼻腔样本和 75% 的活检样本中,至少有一种细菌的培养结果呈阳性。狗最常分离出的细菌种类是假中间葡萄球菌、葡萄球菌属和链球菌属,而猫最常培养出的细菌种类是巴氏杆菌属和猫葡萄球菌属、对于最常见的培养菌种--葡萄球菌属,不同地点之间的平均一致性从未超过一般水平,精确度也有很大差异:本研究表明,猫科动物和犬科动物鼻部疾病的细菌培养结果具有部位特异性,而且本研究没有证据表明同一患者的不同部位对多种细菌的培养结果具有一致性。因此,如果用鼻拭子的细菌培养结果来指导治疗性抗菌药的选择,可能会根据培养部位的不同而选择不同的治疗方法。因此,本研究没有证据表明应建议将鼻腔细菌培养作为常规诊断措施。
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来源期刊
New Zealand veterinary journal
New Zealand veterinary journal 农林科学-兽医学
CiteScore
3.00
自引率
0.00%
发文量
37
审稿时长
12-24 weeks
期刊介绍: The New Zealand Veterinary Journal (NZVJ) is an international journal publishing high quality peer-reviewed articles covering all aspects of veterinary science, including clinical practice, animal welfare and animal health. The NZVJ publishes original research findings, clinical communications (including novel case reports and case series), rapid communications, correspondence and review articles, originating from New Zealand and internationally. Topics should be relevant to, but not limited to, New Zealand veterinary and animal science communities, and include the disciplines of infectious disease, medicine, surgery and the health, management and welfare of production and companion animals, horses and New Zealand wildlife. All submissions are expected to meet the highest ethical and welfare standards, as detailed in the Journal’s instructions for authors.
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