Guarana (Paullinia cupana) as a potential tool for mesenchymal stromal cells priming in regenerative medicine.

IF 16.4 1区化学 Q1 CHEMISTRY, MULTIDISCIPLINARY

Accounts of Chemical Research Pub Date : 2024-07-29 eCollection Date: 2024-01-01 DOI:10.1590/1414-431X2024e13286

D H Sirena, A B Araújo, A B T da Silveira, M A Serafini, M M F da Silva, A K Silveira, E Filippi-Chiela, J C F Moreira, A H Paz

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引用次数: 0

Abstract

Mesenchymal stromal cells (MSCs) have therapeutic potential due to their abilities of differentiation, immunomodulation, and migration to injured tissues, potentiating such effects when cells are activated. Guarana (Paullinia cupana) is a tropical plant species found in South America that is known for its antioxidant, stimulant, and cicatricial effects. The guarana extract is composed of many substances and caffeine is the main component. The objective was to evaluate the effects of guarana and caffeine on MSCs. After the initial characterization, MSCs were treated with Paullinia cupana (10, 100, and 1000 μg/mL) or caffeine (0.4, 4, and 40 μg/mL) for 24 h. MSCs treatment with 1000 μg/mL guarana increased cell polarity, viability, cell migration to chemoattractant, antioxidant potential, and liberation of extracellular vesicles (EVs), while it reduced the levels of autophagy. MSCs treated with 100 and 1000 μg/mL guarana or 40 μg/mL caffeine showed a decrease of cell proliferation. No treatment affected the cellular area and cell cycle of MSCs. The study shows in vitro evidence that guarana could be a promising alternative for activating MSCs to promote better cellular products for future clinical therapies.

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瓜拿纳（Paullinia cupana）作为再生医学中间质基质细胞引物的潜在工具。

间充质基质细胞（MSCs）具有分化、免疫调节和向受伤组织迁移的能力，当细胞被激活时，这些能力会增强治疗效果。瓜拿纳（Paullinia cupana）是一种生长在南美洲的热带植物，以其抗氧化、刺激和止血作用而闻名。瓜拿纳提取物由多种物质组成，咖啡因是其主要成分。研究的目的是评估瓜拿纳和咖啡因对间叶干细胞的影响。在对间叶干细胞进行初步特征描述后，用瓜拉纳（10、100 和 1000 μg/mL）或咖啡因（0.4、4 和 40 μg/mL）处理间叶干细胞 24 小时。1000 μg/mL瓜拉纳处理间叶干细胞可增加细胞极性、活力、细胞向趋化吸引剂迁移、抗氧化潜能和细胞外囊泡（EVs）的释放，同时降低自噬水平。用 100 和 1000 μg/mL 瓜拉纳或 40 μg/mL 咖啡因处理间充质干细胞会导致细胞增殖减少。任何处理都不会影响间充质干细胞的细胞面积和细胞周期。这项研究在体外证明，瓜拉纳可能是激活间充质干细胞的一种有前途的替代品，可促进未来临床疗法中更好的细胞产品。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Accounts of Chemical Research 化学-化学综合

CiteScore

31.40

自引率

1.10%

发文量

312

审稿时长

2 months

期刊介绍： Accounts of Chemical Research presents short, concise and critical articles offering easy-to-read overviews of basic research and applications in all areas of chemistry and biochemistry. These short reviews focus on research from the author’s own laboratory and are designed to teach the reader about a research project. In addition, Accounts of Chemical Research publishes commentaries that give an informed opinion on a current research problem. Special Issues online are devoted to a single topic of unusual activity and significance. Accounts of Chemical Research replaces the traditional article abstract with an article "Conspectus." These entries synopsize the research affording the reader a closer look at the content and significance of an article. Through this provision of a more detailed description of the article contents, the Conspectus enhances the article's discoverability by search engines and the exposure for the research.