{"title":"A comprehensive review on utilizing CRISPR/Cas system for microbiome modification","authors":"","doi":"10.1016/j.bej.2024.109443","DOIUrl":null,"url":null,"abstract":"<div><p>The recent advancements in biotechnology, particularly the clustered regularly interspaced short palindromic repeats (CRISPR)/Cas system inspired by bacterial defense mechanisms, have ushered in a new phase in gene editing. This system is now widely used for a range of purposes, including the diagnosis, treatment, and prevention of various diseases such as genetic conditions, cancer, infectious diseases, and issues related to antibiotic resistance. A particularly notable area of application is the alteration of the human gut microbiota, an essential and complex microbial ecosystem crucial for health. Imbalances in this system referred to as dysbiosis, are associated with several health issues, including heart disease, mental health disorders, and cancer. Therefore, the potential of manipulating microbiota for health benefits is significant. In accordance with this, recent research underscores the influence of modifying specific gut microbiota on the progression of diseases, notably in fields like oncology and inflammation. The current paper examines the impact of CRISPR technology in altering microbiota with the goal of exploiting its capabilities to improve human health. This exploration aims to move beyond theoretical research, applying CRISPR's sophisticated functions to complex and practical health scenarios with the ultimate objective of improving health outcomes.</p></div>","PeriodicalId":8766,"journal":{"name":"Biochemical Engineering Journal","volume":null,"pages":null},"PeriodicalIF":3.7000,"publicationDate":"2024-07-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Biochemical Engineering Journal","FirstCategoryId":"5","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S1369703X24002304","RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"BIOTECHNOLOGY & APPLIED MICROBIOLOGY","Score":null,"Total":0}
引用次数: 0
Abstract
The recent advancements in biotechnology, particularly the clustered regularly interspaced short palindromic repeats (CRISPR)/Cas system inspired by bacterial defense mechanisms, have ushered in a new phase in gene editing. This system is now widely used for a range of purposes, including the diagnosis, treatment, and prevention of various diseases such as genetic conditions, cancer, infectious diseases, and issues related to antibiotic resistance. A particularly notable area of application is the alteration of the human gut microbiota, an essential and complex microbial ecosystem crucial for health. Imbalances in this system referred to as dysbiosis, are associated with several health issues, including heart disease, mental health disorders, and cancer. Therefore, the potential of manipulating microbiota for health benefits is significant. In accordance with this, recent research underscores the influence of modifying specific gut microbiota on the progression of diseases, notably in fields like oncology and inflammation. The current paper examines the impact of CRISPR technology in altering microbiota with the goal of exploiting its capabilities to improve human health. This exploration aims to move beyond theoretical research, applying CRISPR's sophisticated functions to complex and practical health scenarios with the ultimate objective of improving health outcomes.
期刊介绍:
The Biochemical Engineering Journal aims to promote progress in the crucial chemical engineering aspects of the development of biological processes associated with everything from raw materials preparation to product recovery relevant to industries as diverse as medical/healthcare, industrial biotechnology, and environmental biotechnology.
The Journal welcomes full length original research papers, short communications, and review papers* in the following research fields:
Biocatalysis (enzyme or microbial) and biotransformations, including immobilized biocatalyst preparation and kinetics
Biosensors and Biodevices including biofabrication and novel fuel cell development
Bioseparations including scale-up and protein refolding/renaturation
Environmental Bioengineering including bioconversion, bioremediation, and microbial fuel cells
Bioreactor Systems including characterization, optimization and scale-up
Bioresources and Biorefinery Engineering including biomass conversion, biofuels, bioenergy, and optimization
Industrial Biotechnology including specialty chemicals, platform chemicals and neutraceuticals
Biomaterials and Tissue Engineering including bioartificial organs, cell encapsulation, and controlled release
Cell Culture Engineering (plant, animal or insect cells) including viral vectors, monoclonal antibodies, recombinant proteins, vaccines, and secondary metabolites
Cell Therapies and Stem Cells including pluripotent, mesenchymal and hematopoietic stem cells; immunotherapies; tissue-specific differentiation; and cryopreservation
Metabolic Engineering, Systems and Synthetic Biology including OMICS, bioinformatics, in silico biology, and metabolic flux analysis
Protein Engineering including enzyme engineering and directed evolution.