Single-cell RNA profiling of the primary tumor and metastatic lymph node lesions in a patient with clear cell sarcoma of soft tissue: A case report

JOS Case Reports Pub Date : 2025-03-01 Epub Date: 2024-07-24 DOI:10.1016/j.joscr.2024.07.003

Yusuke Tsuda , Hiroyuki Okada , Kotoe Katayama , Toshihide Hirai , Jyunya Miyahara , Liuzhe Zhang , Yasunori Omata , Kasumi Abe , Yutaka Suzuki , Sakae Tanaka , Hiroshi Kobayashi

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Abstract

Background

There was no report which evaluated the cellular constitution and cell–cell interactions in clear cell sarcoma of soft tissue.

Case presentation

A 47-year-old woman underwent surgical excision of clear cell sarcoma in right knee and inguinal lymph node metastasis after neo-adjuvant chemotherapy. However, she died due to liver and para-aortic lymph node metastasis. Single-cell RNA sequencing of the primary tumor and metastatic lymph node samples revealed the accumulation of CTLA4⁺/CD4⁺ regulatory and LAG3⁺/CD8⁺ exhausted T cells particularly in the metastatic lymph node. NicheNet analysis indicated that these T cell states were induced by the interplay of T cells with tumor cells, macrophages, B cells, fibroblasts, and endothelial cell.

Conclusions

The cellular constitution of immune and stromal cell types and their properties suggested the presence of T cell immunosuppression in the clear cell sarcoma microenvironment.

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对一名软组织透明细胞肉瘤患者的原发肿瘤和转移淋巴结病灶进行单细胞 RNA 分析：病例报告

背景软组织透明细胞肉瘤的细胞构成和细胞间相互作用尚未见报道。一例47岁女性患者在新辅助化疗后行右膝透明细胞肉瘤手术切除及腹股沟淋巴结转移。然而，她死于肝脏和主动脉旁淋巴结转移。原发肿瘤和转移淋巴结样本的单细胞RNA测序显示，CTLA4+/CD4+调节性T细胞和LAG3+/CD8+耗竭T细胞在转移淋巴结中富集。NicheNet分析表明，这些T细胞状态是由T细胞与肿瘤细胞、巨噬细胞、B细胞、成纤维细胞和内皮细胞相互作用诱导的。结论透明细胞肉瘤微环境中免疫细胞和基质细胞类型的细胞构成及其性质提示存在T细胞免疫抑制。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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JOS Case Reports

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