{"title":"Boosting of enzymatic cascades by intermediates: Theoretical analysis and model-based optimization","authors":"","doi":"10.1016/j.bej.2024.109440","DOIUrl":null,"url":null,"abstract":"<div><p>A recent paper demonstrated that adding intermediates of an enzymatic cascade reaction at the start of the reaction process can increase the space-time yield significantly. This method was coined boosting by intermediates (BBI) and can potentially increase the productivity of enzymatic cascade reaction systems. BBI remains largely unexplored, and its mechanism, potential, and limitations are not fully understood. In this short communication, the theoretical background of the method is revealed by studying four chemical reaction networks that exhibit boosting through intermediates. The dynamics of these networks are studied via the quasi-steady state approximation and numerical solutions. A novel graphical method that can be used to deduce which intermediates boost linear, redox-neutral enzymatic cascade reaction networks is presented. Further, how such a reaction process can be designed using model-based optimization is demonstrated.</p></div>","PeriodicalId":8766,"journal":{"name":"Biochemical Engineering Journal","volume":null,"pages":null},"PeriodicalIF":3.7000,"publicationDate":"2024-07-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S1369703X24002274/pdfft?md5=ae8b0dd7598fe63017ff89805b6cfe81&pid=1-s2.0-S1369703X24002274-main.pdf","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Biochemical Engineering Journal","FirstCategoryId":"5","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S1369703X24002274","RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"BIOTECHNOLOGY & APPLIED MICROBIOLOGY","Score":null,"Total":0}
引用次数: 0
Abstract
A recent paper demonstrated that adding intermediates of an enzymatic cascade reaction at the start of the reaction process can increase the space-time yield significantly. This method was coined boosting by intermediates (BBI) and can potentially increase the productivity of enzymatic cascade reaction systems. BBI remains largely unexplored, and its mechanism, potential, and limitations are not fully understood. In this short communication, the theoretical background of the method is revealed by studying four chemical reaction networks that exhibit boosting through intermediates. The dynamics of these networks are studied via the quasi-steady state approximation and numerical solutions. A novel graphical method that can be used to deduce which intermediates boost linear, redox-neutral enzymatic cascade reaction networks is presented. Further, how such a reaction process can be designed using model-based optimization is demonstrated.
期刊介绍:
The Biochemical Engineering Journal aims to promote progress in the crucial chemical engineering aspects of the development of biological processes associated with everything from raw materials preparation to product recovery relevant to industries as diverse as medical/healthcare, industrial biotechnology, and environmental biotechnology.
The Journal welcomes full length original research papers, short communications, and review papers* in the following research fields:
Biocatalysis (enzyme or microbial) and biotransformations, including immobilized biocatalyst preparation and kinetics
Biosensors and Biodevices including biofabrication and novel fuel cell development
Bioseparations including scale-up and protein refolding/renaturation
Environmental Bioengineering including bioconversion, bioremediation, and microbial fuel cells
Bioreactor Systems including characterization, optimization and scale-up
Bioresources and Biorefinery Engineering including biomass conversion, biofuels, bioenergy, and optimization
Industrial Biotechnology including specialty chemicals, platform chemicals and neutraceuticals
Biomaterials and Tissue Engineering including bioartificial organs, cell encapsulation, and controlled release
Cell Culture Engineering (plant, animal or insect cells) including viral vectors, monoclonal antibodies, recombinant proteins, vaccines, and secondary metabolites
Cell Therapies and Stem Cells including pluripotent, mesenchymal and hematopoietic stem cells; immunotherapies; tissue-specific differentiation; and cryopreservation
Metabolic Engineering, Systems and Synthetic Biology including OMICS, bioinformatics, in silico biology, and metabolic flux analysis
Protein Engineering including enzyme engineering and directed evolution.