Serum HOTTIP expression is upregulated in nasopharyngeal carcinoma patients and predicts poor prognosis

IF 1.7 4区医学 Q2 OTORHINOLARYNGOLOGY

Brazilian Journal of Otorhinolaryngology Pub Date : 2024-07-20 DOI:10.1016/j.bjorl.2024.101471

Ding-Ting Wang , Jian Luo , Hua-Jun Feng , Yuan-Yuan Wang

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引用次数: 0

Abstract

Objective

Nasopharyngeal Carcinoma (NPC) is a tumor with ethnic and geographic distribution characteristics. HOXA Transcript at the distal Tip (HOTTIP) has been confirmed to have cancer-promoting effects in various tumors. The aim of this study was to investigate the clinical significance of HOTTIP in the development of NPC and its role in prognostic assessment.

Methods

Reverse transcription real-time PCR was used to analyze the expression of HOTTIP in the serum of 122 NPC patients before treatment, 35 controls and 30 NPC patients after treatment, and the relationship between HOTTIP expression and the clinicopathological features and prognosis of NPC patients was analyzed. The NPC cell lines CNE1 and HNE1 stably overexpressing HOTTIP were constructed using the retroviral method. Plate clone formation assays, MTT assays, flow cytometry, and Transwell migration and invasion assays were used to investigate the effects of HOTTIP overexpression on the colony-formation ability, cell proliferation ability, apoptosis, and migration and invasion abilities of CNE1 and HNE1 cells, respectively.

Results

HOTTIP was highly expressed in NPC serum and cells compared with the control group; serum HOTTIP expression in NPC patients after treatment was significantly lower than that before treatment; Kaplan-Meier survival curve revealed that the Progression-Free Survival (PFS) and Overall Survival (OS) of the HOTTIP low-expression group were both better than those of the HOTTIP high-expression group; COX proportional hazard models showed that high HOTTIP expression was an independent risk factor for PFS in NPC patients. The overexpression of HOTTIP promoted the proliferation, migration and invasion abilities of CNE1 and HNE1 NPC cells and inhibited cell apoptosis in vitro cell experiments.

Conclusion

Increased HOTTIP expression in serum indicates a poor prognosis and may be used as a molecular marker and therapeutic target in NPC.

Level of Evidence

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鼻咽癌患者血清 HOTTIP 表达上调，预示预后不良

目的鼻咽癌（NPC）是一种具有种族和地理分布特征的肿瘤。已证实HOXA远端转录蛋白（HOTTIP）在多种肿瘤中具有促癌作用。方法采用反转录实时 PCR 分析 122 例鼻咽癌患者治疗前、35 例对照组和 30 例鼻咽癌患者治疗后血清中 HOTTIP 的表达，并分析 HOTTIP 表达与鼻咽癌患者临床病理特征和预后的关系。采用逆转录病毒方法构建了稳定过表达HOTTIP的鼻咽癌细胞系CNE1和HNE1。采用平板克隆形成试验、MTT试验、流式细胞术和Transwell迁移与侵袭试验分别研究了HOTTIP过表达对CNE1和HNE1细胞的集落形成能力、细胞增殖能力、细胞凋亡和迁移与侵袭能力的影响。结果与对照组相比，HOTTIP在鼻咽癌血清和细胞中高表达；治疗后鼻咽癌患者血清中HOTTIP的表达明显低于治疗前；Kaplan-Meier生存曲线显示，HOTTIP低表达组的无进展生存期（PFS）和总生存期（OS）均优于HOTTIP高表达组；COX比例危险模型显示，HOTTIP高表达是鼻咽癌患者PFS的独立危险因素。在体外细胞实验中，HOTTIP的过表达促进了CNE1和HNE1鼻咽癌细胞的增殖、迁移和侵袭能力，并抑制了细胞凋亡。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Brazilian Journal of Otorhinolaryngology OTORHINOLARYNGOLOGY-

CiteScore

3.00

自引率

0.00%

发文量

205

审稿时长

4-8 weeks

期刊介绍： Brazilian Journal of Otorhinolaryngology publishes original contributions in otolaryngology and the associated areas (cranio-maxillo-facial surgery and phoniatrics). The aim of this journal is the national and international divulgation of the scientific production interesting to the otolaryngology, as well as the discussion, in editorials, of subjects of scientific, academic and professional relevance. The Brazilian Journal of Otorhinolaryngology is born from the Revista Brasileira de Otorrinolaringologia, of which it is the English version, created and indexed by MEDLINE in 2005. It is the official scientific publication of the Brazilian Association of Otolaryngology and Cervicofacial Surgery. Its abbreviated title is Braz J Otorhinolaryngol., which should be used in bibliographies, footnotes and bibliographical references and strips.