An ultrasensitive strip sensor for rapid detection of African swine fever virus

IF 1.2 4区化学 Q4 CHEMISTRY, ANALYTICAL

Chinese Journal of Analytical Chemistry Pub Date : 2024-07-01 DOI:10.1016/j.cjac.2024.100416

Mengjing ZHANG, Lingling GUO, Xinxin XU, Hua KUANG, Chuanlai XU, Liqiang LIU

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Abstract

African swine fever (ASF) is a highly contagious disease caused by the African swine fever virus (ASFV) infecting pigs, which has caused huge economic losses in countries around the world. Currently, there is no effective vaccine, and the prevention and control of ASF is mainly through rapid detection, so it is particularly important to carry identify and develop rapid detection methods for ASFV. In this study, recombinant plasmid PET-28a(+)-p30 was constructed, and the recombinant protein was obtained by inducing expression and Ni⁺ resin affinity column purification. Mice were immunized with recombinant p30 protein, and after three immunizations, ten strains of hybridoma cells that stably secreted monoclonal antibodies (mAbs) against p30 protein were obtained by cell fusion and subcloning. A colloidal gold immunochromatography assay (GICA) based on double antibody sandwich technology was established to screen the paired antibodies, and the trapping and detecting antibodies were mAb-11F11 and mAb-7A8, respectively, with a detection limit of 1 ng/mL, which laid an important material foundation for the early detection of ASF in the future.

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用于快速检测非洲猪瘟病毒的超灵敏条状传感器

非洲猪瘟（African swine fever，ASF）是由非洲猪瘟病毒（African swine fever virus，ASFV）感染猪引起的一种高度传染性疾病，给世界各国造成了巨大的经济损失。目前还没有有效的疫苗，非洲猪瘟的防控主要依靠快速检测，因此鉴定和开发非洲猪瘟病毒的快速检测方法尤为重要。本研究构建了重组质粒PET-28a(+)-p30，通过诱导表达和Ni+树脂亲和柱纯化获得重组蛋白。用重组 p30 蛋白免疫小鼠，经过三次免疫后，通过细胞融合和亚克隆获得了十株能稳定分泌 p30 蛋白单克隆抗体（mAbs）的杂交瘤细胞。建立了基于双抗体夹心技术的胶体金免疫层析技术（GICA）筛选配对抗体，诱捕抗体和检测抗体分别为mAb-11F11和mAb-7A8，检测限均为1 ng/mL，为今后早期检测ASF奠定了重要的物质基础。

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来源期刊

Chinese Journal of Analytical Chemistry 化学-分析化学

CiteScore

3.60

自引率

25.00%

发文量

17223

审稿时长

35 days

期刊介绍： Chinese Journal of Analytical Chemistry(CJAC) is an academic journal of analytical chemistry established in 1972 and sponsored by the Chinese Chemical Society and Changchun Institute of Applied Chemistry, Chinese Academy of Sciences. Its objectives are to report the original scientific research achievements and review the recent development of analytical chemistry in all areas. The journal sets up 5 columns including Research Papers, Research Notes, Experimental Technique and Instrument, Review and Progress and Summary Accounts. The journal published monthly in Chinese language. A detailed abstract, keywords and the titles of figures and tables are provided in English, except column of Summary Accounts. Prof. Wang Erkang, an outstanding analytical chemist, academician of Chinese Academy of Sciences & Third World Academy of Sciences, holds the post of the Editor-in-chief.