{"title":"Metabolic engineering of fast growing cyanobacteria for phototrophic production of 2,3-butanediol","authors":"","doi":"10.1016/j.bej.2024.109439","DOIUrl":null,"url":null,"abstract":"<div><p>Metabolic engineering of cyanobacteria holds great potential for sustainable photosynthetic production of platform chemicals from CO<sub>2</sub>. However, product titers have been significantly low due to the slow growth rates of available model strains and lack of adequate synthetic biology tools. Here, we engineered three newly isolated fast growing <em>Synechococcus elongatus</em> strains PCC 11801, PCC 11802, and IITB6 for production of the platform chemical 2,3-butanediol. Importantly, we used native cyanobacterial promoters to enable inducer-free gene expression and 2,3-butanediol production. Different combinations of these native promoters were employed to optimize expression of the three-gene 2,3-butanediol synthesis pathway. Among the strains tested in this study, the highest 2,3-butanediol titer of 1.62 g L<sup>−1</sup> (130 mg L<sup>−1</sup> Day<sup>−1</sup>) was obtained in IITB6 (<em>P</em><sub><em>cpcB300</em></sub>:<em>alsS</em>::<em>P</em><sub><em>psbAIII</em></sub>:<em>alsD</em>::<em>P</em><sub><em>rbcL</em></sub>:adh), with the highest reported photosynthetic productivity in cyanobacteria cultivated on minimal media. The findings from our study highlight the potential of using fast growing <em>S. elongatus</em> isolates with native cyanobacterial promoters for metabolic engineering applications.</p></div>","PeriodicalId":8766,"journal":{"name":"Biochemical Engineering Journal","volume":null,"pages":null},"PeriodicalIF":3.7000,"publicationDate":"2024-07-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Biochemical Engineering Journal","FirstCategoryId":"5","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S1369703X24002262","RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"BIOTECHNOLOGY & APPLIED MICROBIOLOGY","Score":null,"Total":0}
引用次数: 0
Abstract
Metabolic engineering of cyanobacteria holds great potential for sustainable photosynthetic production of platform chemicals from CO2. However, product titers have been significantly low due to the slow growth rates of available model strains and lack of adequate synthetic biology tools. Here, we engineered three newly isolated fast growing Synechococcus elongatus strains PCC 11801, PCC 11802, and IITB6 for production of the platform chemical 2,3-butanediol. Importantly, we used native cyanobacterial promoters to enable inducer-free gene expression and 2,3-butanediol production. Different combinations of these native promoters were employed to optimize expression of the three-gene 2,3-butanediol synthesis pathway. Among the strains tested in this study, the highest 2,3-butanediol titer of 1.62 g L−1 (130 mg L−1 Day−1) was obtained in IITB6 (PcpcB300:alsS::PpsbAIII:alsD::PrbcL:adh), with the highest reported photosynthetic productivity in cyanobacteria cultivated on minimal media. The findings from our study highlight the potential of using fast growing S. elongatus isolates with native cyanobacterial promoters for metabolic engineering applications.
期刊介绍:
The Biochemical Engineering Journal aims to promote progress in the crucial chemical engineering aspects of the development of biological processes associated with everything from raw materials preparation to product recovery relevant to industries as diverse as medical/healthcare, industrial biotechnology, and environmental biotechnology.
The Journal welcomes full length original research papers, short communications, and review papers* in the following research fields:
Biocatalysis (enzyme or microbial) and biotransformations, including immobilized biocatalyst preparation and kinetics
Biosensors and Biodevices including biofabrication and novel fuel cell development
Bioseparations including scale-up and protein refolding/renaturation
Environmental Bioengineering including bioconversion, bioremediation, and microbial fuel cells
Bioreactor Systems including characterization, optimization and scale-up
Bioresources and Biorefinery Engineering including biomass conversion, biofuels, bioenergy, and optimization
Industrial Biotechnology including specialty chemicals, platform chemicals and neutraceuticals
Biomaterials and Tissue Engineering including bioartificial organs, cell encapsulation, and controlled release
Cell Culture Engineering (plant, animal or insect cells) including viral vectors, monoclonal antibodies, recombinant proteins, vaccines, and secondary metabolites
Cell Therapies and Stem Cells including pluripotent, mesenchymal and hematopoietic stem cells; immunotherapies; tissue-specific differentiation; and cryopreservation
Metabolic Engineering, Systems and Synthetic Biology including OMICS, bioinformatics, in silico biology, and metabolic flux analysis
Protein Engineering including enzyme engineering and directed evolution.